Methods in Molecular Biology

Heterologous Gene Expression in E.coli

Methods and Protocols

Editors: Evans, Jr., Thomas C., Xu, Ming-Qun (Eds.)

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  • Seeks to familiarize the researcher with the myriad of E. coli expression strains available
  • Provides easy to follow protocols, ready to be implemented in the lab
  • Includes tips from the experienced, expert contributors on troubleshooting common methodological pitfalls
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Hardcover $219.99
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Softcover $179.00
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About this book

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Reviews

From the reviews:

“The editors bring together a diverse set of methods and examples demonstrating that, in fact, there is still plenty of active and exiting research pushing back some of the aforementioned limitations of E. coli. … If recombinant protein expression is an important part of your research programme, either in an academic or industrial setting, then this book is worth a read – it could save you a fortune!” (Gavin Thomas, Microbiology Today, November, 2011)

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Adjustment of Codon Usage Frequencies by Codon Harmonization Improves Protein Expression and Folding

    Angov, Evelina (et al.)

    Pages 1-13

  • SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes

    Peroutka III, Raymond J. (et al.)

    Pages 15-30

  • Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins

    Marco, Ario

    Pages 31-51

  • Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System

    Fisher, Adam C. (et al.)

    Pages 53-67

  • Protein Folding Liquid Chromatography

    Bai, Quan (et al.)

    Pages 69-85

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-61737-967-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-61737-966-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5725-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Heterologous Gene Expression in E.coli
Book Subtitle
Methods and Protocols
Editors
  • Thomas C. Evans, Jr.
  • Ming-Qun Xu
Series Title
Methods in Molecular Biology
Series Volume
705
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61737-967-3
DOI
10.1007/978-1-61737-967-3
Hardcover ISBN
978-1-61737-966-6
Softcover ISBN
978-1-4939-5725-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 310
Topics