Methods in Molecular Biology

Nitric Oxide

Methods and Protocols

Editors: McCarthy, Helen O., Coulter, Jonathan A. (Eds.)

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  • Addresses the challenges of accurate quantification of nitric oxide in either in vitro or in vivo models with practical, step-by-step protocols
  • Includes readily reproducible techniques crafted by leading experts
  • Provides detailed notes sections, documenting specific information on particularly challenging aspects of the methodologies
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eBook $109.00
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  • ISBN 978-1-61737-964-2
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Hardcover $149.99
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About this book

The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Nitric Oxide Physiology and Pathology

    Pages 1-13

    Hirst, David G. (et al.)

  • Ionizing Radiation-Induced DNA Strand Breaks and γ-H2AX Foci in Cells Exposed to Nitric Oxide

    Pages 17-25

    Rothkamm, Kai (et al.)

  • Determination of S-Nitrosothiols in Biological Fluids by Chemiluminescence

    Pages 27-37

    Nagababu, Enika (et al.)

  • Measurement of Nitrite in Blood Samples Using the Ferricyanide-Based Hemoglobin Oxidation Assay

    Pages 39-56

    Piknova, Barbora (et al.)

  • Selective Fluorescent Activation for Bioimaging the Expression of Nitric Oxide in Cellular and In Vivo Systems

    Pages 57-71

    Zhang, Junfeng (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-61737-964-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
Softcover $149.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Nitric Oxide
Book Subtitle
Methods and Protocols
Editors
  • Helen O. McCarthy
  • Jonathan A. Coulter
Series Title
Methods in Molecular Biology
Series Volume
704
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61737-964-2
DOI
10.1007/978-1-61737-964-2
Hardcover ISBN
978-1-61737-963-5
Softcover ISBN
978-1-4939-5791-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 226
Topics