Save 40% on select Business & Management books + FREE shipping or 50% on Physics eBooks!

Methods in Molecular Biology

Amyloid Proteins

Editors: Sigurdsson, Einar M. (Ed.)

Free Preview

Buy this book

eBook $99.00
price for USA in USD (gross)
  • ISBN 978-1-59259-874-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $134.00
price for USA in USD
  • ISBN 978-1-61737-502-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In recent years, various in vitro and in vivo techniques, transgenic technologies, and novel animal models have been developed to understand amyloid diseases-such as Alzheimer's disease and some forms of diabetes-and to screen for drugs that can block amyloid formation and/or disassemble its associated fibrils. In Amyloid Proteins: Methods and Protocols, internationally known researchers describe in step-by-step detail their best techniques for studying these types of proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide proven methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Amyloid Proteins: Methods and Protocols offers both skilled and novice investigators a proven collection of standard and novel techniques for studying amyloid proteins and peptides, as well as their relation to amyloid diseases.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Preparation of Aggregate-Free,Low Molecular Weight Amyloidβ for Assembly and Toxicity Assays

    Pages 3-9

    Bitan, Gal (et al.)

  • Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking

    Pages 11-18

    Vollers, Sabrina S. (et al.)

  • In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α Synuclein

    Pages 19-33

    Lashuel, Hilal A. (et al.)

  • Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro

    Pages 35-51

    Barghorn, Stefan (et al.)

  • Cyclic Amplification of Protein Misfolding and Aggregation

    Pages 53-65

    Saá, Paula (et al.)

Buy this book

eBook $99.00
price for USA in USD (gross)
  • ISBN 978-1-59259-874-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $134.00
price for USA in USD
  • ISBN 978-1-61737-502-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Amyloid Proteins
Editors
  • Einar M. Sigurdsson
Series Title
Methods in Molecular Biology
Series Volume
299
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-874-8
DOI
10.1385/1592598749
Softcover ISBN
978-1-61737-502-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
408
Number of Illustrations
155 b/w illustrations, 1 illustrations in colour
Topics