Methods in Molecular Biology

Vaccinia Virus and Poxvirology

Methods and Protocols

Editors: Isaacs, Stuart N. (Ed.)

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About this book

Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents.

Reviews

From the foreword...

"The right book at the right time." -Bernard Moss

"...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapy

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Working Safely with Vaccinia Virus

    Pages 1-13

    Isaacs, Stuart N.

  • Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers

    Pages 15-30

    Lorenzo, Maríia M. (et al.)

  • Construction of Recombinant Vaccinia Virus

    Pages 31-40

    Byrd, Chelsea M. (et al.)

  • Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses

    Pages 41-50

    Mohamed, Mohamed Ragaa (et al.)

  • Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA

    Pages 51-64

    Yao, Xiao-Dan (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-789-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $139.00
price for USA in USD
  • ISBN 978-1-61737-437-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Vaccinia Virus and Poxvirology
Book Subtitle
Methods and Protocols
Editors
  • Stuart N. Isaacs
Series Title
Methods in Molecular Biology
Series Volume
269
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-789-5
DOI
10.1385/1592597890
Softcover ISBN
978-1-61737-437-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XX, 396
Topics