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Mesenchymal Stem Cell Assays and Applications

  • Book
  • © 2011

Overview

Editors:
  1. Mohan Vemuri

    1. , Primary and Stem Cell Systems, Life Technologies, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Lucas G. Chase

    1. Cellular Dynamics International, Inc., Madison, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Mahendra S. Rao

    1. Life Technologies, Frederick, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes thirty-five detailed chapters from leading experts around the world
  • Describes cutting-edge methods to isolate, propagate, characterize, and engineer mesenchymal stem cells
  • Highlights a set of optimized protocols to facilitate the use of standardized methods throughout the mesenchymal stem cell research field
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 698)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Mesenchymal Stem Cell Assays and Applications

      • Mohan C. Vemuri, Lucas G. Chase, Mahendra S. Rao
      Pages 3-8

  3. Isolation and Expansion of MSCs from Various Sources

    1. Front Matter

      Pages 9-9
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    2. Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow

      • Patrice Penfornis, Radhika Pochampally
      Pages 11-21

    3. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis

      • Patrick Horn, Simone Bork, Wolfgang Wagner
      Pages 23-35

    4. Isolation and Growth of Adipose Tissue-Derived Stem Cells

      • Vladimir Zachar, Jeppe Grøndahl Rasmussen, Trine Fink
      Pages 37-49

    5. Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells

      • Alp Can, Deniz Balci
      Pages 51-62

    6. The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions

      • Anita Laitinen, Johanna Nystedt, Saara Laitinen
      Pages 63-73

    7. Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture

      • Justin D. Klein, Dario O. Fauza
      Pages 75-88

    8. Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells

      • Nina Ilic, Gary Brooke, Patricia Murray, Sarah Barlow, Tony Rossetti, Rebecca Pelekanos et al.
      Pages 89-106

    9. A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells

      • Stan Gronthos, Agnieszka Arthur, P. Mark Bartold, Songtao Shi
      Pages 107-121

    10. Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas

      • Karen L. Seeberger, Alana Eshpeter, Gregory S. Korbutt
      Pages 123-140

    11. Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells

      • Ruenn Chai Lai, Andre Choo, Sai Kiang Lim
      Pages 141-150

    12. Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells

      • Nance Beyer Nardi, Melissa Camassola
      Pages 151-160

    13. Cryopreservation and Revival of Mesenchymal Stromal Cells

      • Mandana Haack-Sørensen, Jens Kastrup
      Pages 161-174

    14. Dynamic Expansion Culture for Mesenchymal Stem Cells

      • Hicham Majd, Thomas M. Quinn, Pierre-Jean Wipff, Boris Hinz
      Pages 175-188

    15. Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers

      • Francisco dos Santos, Pedro Z. Andrade, Gemma Eibes, Cláudia Lobato da Silva, Joaquim M. S. Cabral
      Pages 189-198

  4. MSC Lineage Differentiation and Analysis

    1. Front Matter

      Pages 199-199
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    2. Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells

      • Deepak M. Gupta, Nicholas J. Panetta, Michael T. Longaker
      Pages 201-214
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Keywords

About this book

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Editors and Affiliations

  • , Primary and Stem Cell Systems, Life Technologies, Frederick, USA

    Mohan Vemuri

  • Cellular Dynamics International, Inc., Madison, USA

    Lucas G. Chase

  • Life Technologies, Frederick, USA

    Mahendra S. Rao

Bibliographic Information

  • Book Title: Mesenchymal Stem Cell Assays and Applications

  • Editors: Mohan Vemuri, Lucas G. Chase, Mahendra S. Rao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-999-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science + Business Media, LLC 2011

  • Hardcover ISBN: 978-1-60761-998-7Published: 06 April 2011

  • Softcover ISBN: 978-1-4939-5779-8Published: 04 May 2017

  • eBook ISBN: 978-1-60761-999-4Published: 23 March 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 534

  • Topics: Cell Biology, Stem Cells

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