Transgenic Mouse Methods and Protocols

1. Front Matter

   Pages i-xi

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2. Introduction: Strategies for Developing Genetically Modified Mice

   • Marten H. Hofker

   Pages 1-10

3. Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection

   • Jan Willem Voncken

   Pages 11-36

4. Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos

   • Jérôme Artus, Anna-Katerina Hadjantonakis

   Pages 37-56

5. Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

   • Naomi Nakagata

   Pages 57-73

6. Autopsy and Histological Analysis of the Transgenic Mouse

   • Marion J. J. Gijbels, Menno P. J. de Winther

   Pages 75-87

7. Transgene Design

   • Bart van de Sluis, Jan Willem Voncken

   Pages 89-101

8. Inducible Transgenic Mouse Models

   • Thomas L. Saunders

   Pages 103-115

9. Lentiviral Transgenesis

   • Terunaga Nakagawa, Casper C. Hoogenraad

   Pages 117-142

10. Transgenesis in Mouse Embryonic Stem Cells

    • Janine van Ree, Wei Zhou, Ming Li, Jan M. van Deursen

    Pages 143-162

11. Engineering BAC Reporter Gene Constructs for Mouse Transgenesis

    • Yu Fu, Peter Maye

    Pages 163-179

12. Targeting Vector Construction Through Recombineering

    • Liviu A. Malureanu

    Pages 181-203

13. Generating Conditional Knockout Mice

    • Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers, Ralf Kühn

    Pages 205-231

14. Hypomorphic Mice

    • Darren J. Baker

    Pages 233-244

15. MICER Targeting Vectors for Manipulating the Mouse Genome

    • Chunhong Liu, Paul F. Szurek, Y. Eugene Yu

    Pages 245-256

16. Knock-In Approaches

    • Anton J. M. Roebroek, Philip L. S. M. Gordts, Sara Reekmans

    Pages 257-275

17. Generation of a Series of Knock-In Alleles Using RMCE in ES Cells

    • Anton J. M. Roebroek, Philip L. S. M. Gordts, Sara Reekmans

    Pages 277-281

18. Selection of Targeted Mutants from a Library of Randomly Mutagenized ES Cells

    • Kyoji Horie, George Gaitanaris, Alexander Gragerov

    Pages 283-294

19. Generation of Genetically Modified Rodents Using Random ENU Mutagenesis

    • Ruben van Boxtel, Edwin Cuppen

    Pages 295-308

20. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

    • Menno P. J. de Winther, Peter Heeringa

    Pages 309-320

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

• Gene function
• Genotype-phenotype relationships
• Knockout mice
• Transgenic mice
• Transgenic mouse models
• genetic engineering

From the reviews of the second edition:

“Provided us with the valuable second edition of Transgenic mouse … . A great merit of this book is then the fact that the two Editors decided to devote some chapters to this basic topic! … I strongly suggest to have a copy of this book to those would like to enter the fascinating field of transgenesis; those already active in the field will anyhow surely find interesting aspects to deal with by comparing the techniques they are using with the suggested novelties here presented.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

• Dept. Pathology & Medical Biology, Medical Biology Section, University Medical Center Groningen, Groningen, Netherlands

  Marten H. Hofker

• Mayo Clinic, Rochester, USA

  Jan Deursen

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