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Methods in Molecular Biology

Transgenic Mouse Methods and Protocols

Editors: Hofker, Marten H., Van Deursen, Jan (Eds.)

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  • Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models
  • Provides descriptions of how to generate conventional knockout and transgenic mice
  • Includes detailed protocols for vector preparation of different kinds of mouse models
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eBook $139.00
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  • ISBN 978-1-60761-974-1
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60761-973-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5676-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

Reviews

From the reviews of the second edition:

“Provided us with the valuable second edition of Transgenic mouse … . A great merit of this book is then the fact that the two Editors decided to devote some chapters to this basic topic! … I strongly suggest to have a copy of this book to those would like to enter the fascinating field of transgenesis; those already active in the field will anyhow surely find interesting aspects to deal with by comparing the techniques they are using with the suggested novelties here presented.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Introduction: Strategies for Developing Genetically Modified Mice

    Pages 1-10

    Hofker, Marten H.

  • Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection

    Pages 11-36

    Voncken, Jan Willem

  • Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos

    Pages 37-56

    Artus, Jérôme (et al.)

  • Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

    Pages 57-73

    Nakagata, Naomi

  • Autopsy and Histological Analysis of the Transgenic Mouse

    Pages 75-87

    Gijbels, Marion J. J. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60761-974-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60761-973-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4939-5676-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Transgenic Mouse Methods and Protocols
Editors
  • Marten H. Hofker
  • Jan Van Deursen
Series Title
Methods in Molecular Biology
Series Volume
693
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science + Business Media, LLC
eBook ISBN
978-1-60761-974-1
DOI
10.1007/978-1-60761-974-1
Hardcover ISBN
978-1-60761-973-4
Softcover ISBN
978-1-4939-5676-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 352
Topics