Methods in Molecular Biology

Protein Chromatography

Methods and Protocols

Editors: Walls, Dermot, Loughran, Sinead (Eds.)

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  • Features contributions from experienced scientists with hands-on expertise in the field
  • Presents step-by-step methodologies, tips and associated explanatory notes
  • Includes a large section on the generation and purification of recombinant proteins
  • Addresses protein biochemistry with clear, step-by-step techniques
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eBook $149.00
price for USA in USD
  • ISBN 978-1-60761-913-0
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Hardcover $199.00
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Softcover $199.00
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About this book

A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Table of contents (30 chapters)

Table of contents (30 chapters)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-60761-913-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
Softcover $199.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Protein Chromatography
Book Subtitle
Methods and Protocols
Editors
  • Dermot Walls
  • Sinead Loughran
Series Title
Methods in Molecular Biology
Series Volume
681
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-913-0
DOI
10.1007/978-1-60761-913-0
Hardcover ISBN
978-1-60761-912-3
Softcover ISBN
978-1-4939-6129-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 527
Topics