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Methods in Molecular Biology

Enzyme Stabilization and Immobilization

Methods and Protocols

Editors: Minteer, Shelley D. (Ed.)

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  • -Provides protocols for enzyme stabilization that can be used for any enzyme of interest to the reader
  • -Includes up-to-date protocols on the latest enzyme immobilization techniques
  • -Provides a thorough review of methods for accurately determining enzyme activity and stability
  • -Procedures are scaleable from microscale molecular biology assays to industrial bioprocessing
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eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-895-9
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $169.99
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Softcover $169.99
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About this book

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Introduction to the Field of Enzyme Immobilization and Stabilization

    Pages 1-7

    Moehlenbrock, Michael J. (et al.)

  • Stabilization of Enzymes Through Encapsulation in Liposomes

    Pages 9-18

    Yoshimoto, Makoto

  • Micellar Enzymology for Thermal, pH, and Solvent Stability

    Pages 19-24

    Minteer, Shelley D.

  • Lipase Activation and Stabilization in Room-Temperature Ionic Liquids

    Pages 25-35

    Kaar, Joel L.

  • Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems

    Pages 37-48

    Buthe, Andreas (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-895-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Enzyme Stabilization and Immobilization
Book Subtitle
Methods and Protocols
Editors
  • Shelley D. Minteer
Series Title
Methods in Molecular Biology
Series Volume
679
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-895-9
DOI
10.1007/978-1-60761-895-9
Hardcover ISBN
978-1-60761-894-2
Softcover ISBN
978-1-4939-5792-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 229
Topics