- Expert TMA protocols for all types of tissues including formalin fixed tissues, frozen tissues, and cell suspensions
- Comprehensive description of in-situ techniques at the DNA, RNA, and protein level
- Includes strategies for both manual and automated TMA analysis
Buy this book
- About this book
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Today’s tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.
- Reviews
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From the reviews:
“This book is the first … to attempt to summarise the field of Tissue Microarrays (TMAs) since the first report of the technology in 1998. … relevant to the clinician-scientist who may be considering high-throughput analyses of genes and proteins from a large cohort. … In summary, this book is crammed with incredibly detailed technical advice … and even advice on whether to drill or cut the recipient paraffin blocks. … very readable, very informative and an excellent introduction to the gentle art of TMAs.” (Cris dos Remedios and Sean Lal, Proteomics, Vol. 11, 2011)
- Table of contents (17 chapters)
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Applications of Tissue Microarray Technology
Pages 1-16
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Quality Aspects of TMA Analysis
Pages 17-26
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Representativity of TMA Studies
Pages 27-35
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Recipient Block TMA Technique
Pages 37-44
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Protocol for Constructing Tissue Arrays by Cutting Edge Matrix Assembly
Pages 45-52
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Table of contents (17 chapters)
- Download Preface 1 PDF (1.4 MB)
- Download Sample pages 1 PDF (1.7 MB)
- Download Table of contents PDF (1.4 MB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Tissue Microarrays
- Book Subtitle
- Methods and Protocols
- Editors
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- Ronald Simon
- Series Title
- Methods in Molecular Biology
- Series Volume
- 664
- Copyright
- 2010
- Publisher
- Humana Press
- Copyright Holder
- Springer Science + Business Media, LLC
- eBook ISBN
- 978-1-60761-806-5
- DOI
- 10.1007/978-1-60761-806-5
- Hardcover ISBN
- 978-1-60761-805-8
- Softcover ISBN
- 978-1-4939-6103-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 193
- Topics
