Methods in Molecular Biology

Immunoelectron Microscopy

Methods and Protocols

Editors: Schwartzbach, Steven D., Osafune, Tetsuaki (Eds.)

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  • Presents a wide range of Cryo and chemical fixation methods for single cells, plant, and animal tissue 
  • Contains Immunogold labeling methods for transmission and scanning electron microscopy
  • Includes comprehensive pre and post embedding immunolabeling methods
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Hardcover $199.00
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Softcover $199.00
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About this book

Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.

Reviews

From the reviews:

“The editors tell us that Immunoelectron microscopy is the technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. Immunoelectron microscopy can be used on virtually every unicellular and multicellular organism. … An excellent source of information about the minutiae of preparation techniques if this is your area.” (P. W. Hawles, Ultramicroscopy, Vol. 111 (7), June, 2011)

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Protein Antigen Expression in Escherichia coli for Antibody Production

    Pages 3-20

    Rancour, David M. (et al.)

  • Expression of Epitope-Tagged Proteins in Plants

    Pages 21-31

    Furuichi, Takuya

  • Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts

    Pages 33-42

    Cho, Young-Hee (et al.)

  • Transient Expression of Epitope-Tagged Proteins in Mammalian Cells

    Pages 43-61

    Styers, Melanie L. (et al.)

  • Production and Purification of Polyclonal Antibodies

    Pages 63-74

    Nakazawa, Masami (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-60761-783-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
  • ISBN 978-1-60761-782-2
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $199.00
price for USA in USD
  • ISBN 978-1-4939-5758-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions & severe weather in the US may cause delays
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Immunoelectron Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Steven D. Schwartzbach
  • Tetsuaki Osafune
Series Title
Methods in Molecular Biology
Series Volume
657
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-783-9
DOI
10.1007/978-1-60761-783-9
Hardcover ISBN
978-1-60761-782-2
Softcover ISBN
978-1-4939-5758-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 352
Topics