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Methods in Molecular Biology

LC-MS/MS in Proteomics

Methods and Applications

Editors: Cutillas, Pedro R., Timms, John F. (Eds.)

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  • Examines current LC-MS technologies, instrumentation, applications, and analysis solutions used in quantitative proteomics
  • Details protocols for isobaric tagging, in vivo stable isotopic labelling, label-free quantification, and multiple reaction monitoring
  • Covers the analysis of protein post-translational modifications, protein complexes, organelles, and kinase activities
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60761-780-8
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60761-779-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-5744-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.

Table of contents (21 chapters)

Table of contents (21 chapters)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60761-780-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60761-779-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-5744-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
LC-MS/MS in Proteomics
Book Subtitle
Methods and Applications
Editors
  • Pedro R. Cutillas
  • John F. Timms
Series Title
Methods in Molecular Biology
Series Volume
658
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-780-8
DOI
10.1007/978-1-60761-780-8
Hardcover ISBN
978-1-60761-779-2
Softcover ISBN
978-1-4939-5744-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 357
Topics