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Methods in Molecular Biology

RNAi and microRNA-Mediated Gene Regulation in Stem Cells

Methods, Protocols, and Applications

Editors: Zhang, Baohong, Stellwag, Edmund J. (Eds.)

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  • Includes methods for state-of-the-art deep sequencing, miRNA-based shRNA-induced gene knockdown and qRT-PCR technologies
  • Provides protocols to identify and analyze the expression and function of RNAi and miRNA in stem cells
  • Authoritative guide to the experimental study of the nexus between RNAi and microRNA-mediated control of stem cell function
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eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-769-3
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $169.99
price for USA in USD
About this book

Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination. In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic. Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • RNAi in Stem Cells: Current Status and Future Perspectives

    Pages 3-14

    Zou, Gang-Ming

  • A Brief Introduction to RNAi and MicroRNAs in Stem Cells

    Pages 15-25

    Murashov, Alexander K.

  • Forward RNAi Screens in Human Stem Cells

    Pages 29-43

    Karlsson, Christine (et al.)

  • A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells

    Pages 45-63

    Trombly, Melanie I. (et al.)

  • Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells

    Pages 65-74

    Cheng, Xiaoxing (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-769-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
RNAi and microRNA-Mediated Gene Regulation in Stem Cells
Book Subtitle
Methods, Protocols, and Applications
Editors
  • Baohong Zhang
  • Edmund J. Stellwag
Series Title
Methods in Molecular Biology
Series Volume
650
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-769-3
DOI
10.1007/978-1-60761-769-3
Hardcover ISBN
978-1-60761-768-6
Softcover ISBN
978-1-4939-5749-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 261
Topics