Overview
- Includes methods for state-of-the-art deep sequencing, miRNA-based shRNA-induced gene knockdown and qRT-PCR technologies
- Protocols to identify and analyze the expression and function of RNAi and miRNA in stem cells
- Authoritative guide to the experimental study of the nexus between RNAi and microRNA-mediated control of stem cell function
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 650)
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Keywords
Table of contents (18 protocols)
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Introduction
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RNA Interference
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MicroRNAs
Editors and Affiliations
Bibliographic Information
Book Title: RNAi and microRNA-Mediated Gene Regulation in Stem Cells
Book Subtitle: Methods, Protocols, and Applications
Editors: Baohong Zhang, Edmund J. Stellwag
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-769-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60761-768-6Published: 16 August 2010
Softcover ISBN: 978-1-4939-5749-1Published: 23 August 2016
eBook ISBN: 978-1-60761-769-3Published: 05 August 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 261
Topics: Cell Biology, Biochemistry, general, Human Genetics, Stem Cells, Gene Therapy