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Methods in Molecular Biology

Plant Secondary Metabolism Engineering

Methods and Applications

Editors: Fett-Neto, Arthur Germano (Ed.)

  • -Provides an easily accessible reference source of strategies for engineering plant secondary metabolic pathways
  • -Details the methods to introduce, manipulate, up and down regulate, and evaluate the expression of plant metabolism genes in both homologous and heterologous systems
  • -Supplies practical knowledge toestablish viable systems for the demand of natural products as pharmaceuticals, food additives, nutraceuticals, and special chemicals
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60761-723-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60761-722-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5735-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. In Plant Secondary Metabolism Engineering: Methods and Applications, expert researchers provide detailed practical information on some of the most important methods employed in the engineering of plant secondary metabolism pathways and in the acquisition of essential knowledge in performing this activity, including the significant advances and emerging strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Applications will aid scientists engaged in the challenging task of modifying some of the most intricate products of plant evolution and support their efforts directed toward the vital goal of sustainable natural chemicals.

Table of contents (21 chapters)

  • Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview

    Nascimento, Naíla Cannes do (et al.)

    Pages 1-13

  • Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin

    Ban, Yusuke (et al.)

    Pages 15-31

  • Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System

    Yamada, Yasuyuki (et al.)

    Pages 33-45

  • Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis

    Pattanaik, Sitakanta (et al.)

    Pages 47-57

  • Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid

    Endt, Débora Vom (et al.)

    Pages 59-75

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60761-723-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60761-722-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-5735-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Secondary Metabolism Engineering
Book Subtitle
Methods and Applications
Editors
  • Arthur Germano Fett-Neto
Series Title
Methods in Molecular Biology
Series Volume
643
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-723-5
DOI
10.1007/978-1-60761-723-5
Hardcover ISBN
978-1-60761-722-8
Softcover ISBN
978-1-4939-5735-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 339
Topics