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  • © 2010

Plant Secondary Metabolism Engineering

Methods and Applications

  • Provides an easily accessible reference source of strategies for engineering plant secondary metabolic pathways
  • Details the methods to introduce, manipulate, up and down regulate, and evaluate the expression of plant metabolism genes in both homologous and heterologous systems
  • Supplies practical knowledge toestablish viable systems for the demand of natural products as pharmaceuticals, food additives, nutraceuticals, and special chemicals
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 643)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview

    • Naíla Cannes do Nascimento, Arthur Germano Fett-Neto
    Pages 1-13
  3. Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System

    • Yasuyuki Yamada, Nobuhiko Kato, Yasuhisa Kokabu, Qingyun Luo, Joseph Gogo Dubouzet, Fumihiko Sato
    Pages 33-45
  4. Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity

    • Tomoya Takemura, Yit-lai Chow, Takehiko Todokoro, Takuya Okamoto, Fumihiko Sato
    Pages 95-109
  5. Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties

    • Hiromichi Minami, Nobuhiro Ikezawa, Fumihiko Sato
    Pages 111-120
  6. Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture

    • Hisashi Semba, Eita Ichige, Tadayuki Imanaka, Haruyuki Atomi, Hideki Aoyagi
    Pages 133-144
  7. USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories

    • Hussam H. Nour-Eldin, Fernando Geu-Flores, Barbara A. Halkier
    Pages 185-200
  8. Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants

    • Tomohisa Hasunuma, Akihiko Kondo, Chikahiro Miyake
    Pages 213-227
  9. Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes

    • Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik
    Pages 229-242
  10. Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering

    • Vishal Gaurav, Martin E. Kolewe, Susan C. Roberts
    Pages 243-262

About this book

Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. In Plant Secondary Metabolism Engineering: Methods and Applications, expert researchers provide detailed practical information on some of the most important methods employed in the engineering of plant secondary metabolism pathways and in the acquisition of essential knowledge in performing this activity, including the significant advances and emerging strategies. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Applications will aid scientists engaged in the challenging task of modifying some of the most intricate products of plant evolution and support their efforts directed toward the vital goal of sustainable natural chemicals.

Editors and Affiliations

  • Rio Grande do Sul, Centro de Biotecnologia, Universidade Federal do, Porto Alegre, Brazil

    Arthur Germano Fett-Neto

Bibliographic Information

  • Book Title: Plant Secondary Metabolism Engineering

  • Book Subtitle: Methods and Applications

  • Editors: Arthur Germano Fett-Neto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-723-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-722-8Published: 06 May 2010

  • Softcover ISBN: 978-1-4939-5735-4Published: 23 August 2016

  • eBook ISBN: 978-1-60761-723-5Published: 16 June 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 339

  • Topics: Biotechnology, Plant Sciences, Plant Genetics and Genomics, Gene Expression

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access