Methods in Molecular Biology

Surface Plasmon Resonance

Methods and Protocols

Editors: de Mol, Nico J., Fischer, Marcel J. E. (Eds.)

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  • Provides detailed protocols for a wide variety of SPR applications
  • Includes comprehensive background information on SPR techniques for better experimental design
  • Examines a variety of coupling techniques on different sensor surfaces
  • Highlights background issues involved in SPR assays to avoid common errors frequently found in SPR literature
  • Presents protocols in clear step by step procedures including the basics of kinetic analysis
  • Focuses on a wide range of applications for membrane-bound protein interactions, including G protein-coupled receptors
  • Chapters on the combination of SPR with mass spectrometry (SPR-MS) for quantification and identification of proteins
  • Written for users of commercially available SPR equipment
  • Covers the two most common sources of deviation from ideal kinetics: mass transport and surface heterogeneity
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eBook $129.00
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  • ISBN 978-1-60761-670-2
  • Digitally watermarked, DRM-free
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Hardcover $199.99
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About this book

While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal.  In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Surface Plasmon Resonance: A General Introduction

    Pages 1-14

    Mol, Nico J. (et al.)

  • The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing

    Pages 15-54

    Schuck, Peter (et al.)

  • Amine Coupling Through EDC/NHS: A Practical Approach

    Pages 55-73

    Fischer, Marcel J. E.

  • High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies

    Pages 75-90

    Hutsell, Stephanie Q. (et al.)

  • A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance

    Pages 91-100

    Kimple, Adam J. (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-670-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $199.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Surface Plasmon Resonance
Book Subtitle
Methods and Protocols
Editors
  • Nico J. de Mol
  • Marcel J. E. Fischer
Series Title
Methods in Molecular Biology
Series Volume
627
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-670-2
DOI
10.1007/978-1-60761-670-2
Hardcover ISBN
978-1-60761-669-6
Softcover ISBN
978-1-4939-6125-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 286
Topics