Overview
- Collects in one single volume the most relevant RNA-based technologies, including ribozymes, RNA aptamers, and siRNAs, and mRNA-encoding therapeutic proteins
- Features the translation of RNA-based therapies into the clinic
- Includes techniques to isolate, quantify, and clone small RNAs as well as strategies to overcome siRNA unwanted effects
- Showcases innovative nanotechnologies for RNA delivery and innovative mRNA transfected-DC vaccines
- Provides protocols for manufacturing non-MHC restricted T cells
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 629)
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Table of contents (30 protocols)
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Front Matter
Keywords
About this book
Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes.
Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.
Reviews
From the reviews:
“Give us an update statement of the present day possible use of RNA to treat several diseases are well presented thanks to a 30 chapters walk in the RNA world. … Clearly enough, the book is of great interest to the entire scientific community, no matter if basic researchers, teachers or clinicians!” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)Editors and Affiliations
Bibliographic Information
Book Title: RNA Therapeutics
Book Subtitle: Function, Design, and Delivery
Editors: Mouldy Sioud
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-657-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2010
Hardcover ISBN: 978-1-60761-656-6Published: 16 September 2010
Softcover ISBN: 978-1-4939-6151-1Published: 23 August 2016
eBook ISBN: 978-1-60761-657-3Published: 12 April 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 526
Topics: Biochemistry, general, Human Genetics, Genetic Engineering, Animal Genetics and Genomics, Gene Therapy