CYBER DEAL: 50% off all Springer eBooks | Get this offer!

Methods in Molecular Biology

In Vitro Mutagenesis Protocols

Third Edition

Editors: Braman, Jeff (Ed.)

Free Preview
  • Establishes protein structure, function, activity, and interrelationships by authors of peer reviewed articles published in respected journals
  • Covers computational methods to evaluate protein stability and the resultant change upon introducing mutations
  • Utilizes animal models to identify loci that genetically interact with human disease-causing point mutations
  • Includes detailed methods on protein structure and function mutagenesis
see more benefits

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-652-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
About this book

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.

Reviews

From the reviews of the third edition:

“This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. … Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. … This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area.” (Omer Iqbal, Doody’s Review Service, November, 2010)

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension

    Pages 3-14

    Alcalde, Miguel

  • In Vitro Mutagenesis of Brucella Species

    Pages 15-35

    Ficht, Thomas A. (et al.)

  • Random Mutagenesis Strategies for Campylobacter and Helicobacter Species

    Pages 37-52

    Gaskin, Duncan J. H. (et al.)

  • Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes

    Pages 53-74

    Zhao, Yuguang (et al.)

  • An Efficient Protocol for VZV BAC-Based Mutagenesis

    Pages 75-86

    Zhang, Zhen (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-60761-652-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
In Vitro Mutagenesis Protocols
Book Subtitle
Third Edition
Editors
  • Jeff Braman
Series Title
Methods in Molecular Biology
Series Volume
634
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-652-8
DOI
10.1007/978-1-60761-652-8
Hardcover ISBN
978-1-60761-651-1
Softcover ISBN
978-1-4939-5666-1
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIV, 442
Topics