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Methods in Molecular Biology

Heterologous Expression of Membrane Proteins

Methods and Protocols

Editors: Mus-Veteau, Isabelle (Ed.)

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  • Provides descriptions of existing heterologous expression systems for membrane proteins and instructions for manipulating these systems
  • Contains methods for the purification of membrane proteins
  • Includes information on classical detergents and alternatives for studying membrane proteins in solution
  • Offers methods to study and increase membrane protein’s stability
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eBook $109.00
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  • ISBN 978-1-60761-344-2
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-60761-343-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
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  • ISBN 978-1-4939-6132-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins: Methods and Protocols, expert researchers provide an overview of the different heterologous expression systems available to produce these proteins for structural analysis, helping us to understand how these proteins function, and additionally, how their functions can be modified through drug treatment. Chapters examine membrane protein solubilization, purification, and instability in solution, and investigate the strategies that helped to determine the structure of the first heterologously expressed mammalian membrane proteins. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Far-reaching and innovative, Heterologous Expression of Membrane Proteins: Methods and Protocols, is an essential guide for novice and experienced researchers alike, providing detailed methods for existing expression systems alongside cutting-edge strategies for future use in the field. 

Reviews

From the reviews:

“Well-illustrated book provides an overview of different heterologous expression systems available to produce membrane proteins for structural analysis to facilitate understanding of how these proteins function and how their functions can be modified by drug treatment. … Written for students and experienced researchers, the book also will be useful for clinicians interested in the care of patients with cancer … . provides valuable information on membrane proteins for novice and experienced researchers. Clinicians will find it extremely useful for developing methods and protocols for research.” (Omer Iqbal, Doody’s Review Service, July, 2010)

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Heterologous Expression of Membrane Proteins for Structural Analysis

    Pages 1-16

    Mus-Veteau, Isabelle, Ph.D.

  • Production of Membrane Proteins in Escherichia coli and Lactococcus lactis

    Pages 17-38

    Geertsma, Eric R. (et al.)

  • Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli

    Pages 39-48

    Mouillac, Bernard (et al.)

  • Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies

    Pages 49-66

    Zoonens, Manuela (et al.)

  • Membrane Protein Expression in Lactococcus lactis

    Pages 67-85

    Frelet-Barrand, Annie (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-60761-344-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-60761-343-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-6132-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Heterologous Expression of Membrane Proteins
Book Subtitle
Methods and Protocols
Editors
  • Isabelle Mus-Veteau
Series Title
Methods in Molecular Biology
Series Volume
601
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-344-2
DOI
10.1007/978-1-60761-344-2
Hardcover ISBN
978-1-60761-343-5
Softcover ISBN
978-1-4939-6132-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 272
Topics