Methods in Molecular Biology

Chemotaxis

Methods and Protocols

Editors: Jin, Tian, Hereld, Dale (Eds.)

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  • Describes a breadth of uniquely-suited model systems for analysis of chemotaxis, including bacteria, Dictyostelium, yeast, Drosophila, zebrafish, neurons, and immune and tumor cells
  • Examines phenomena related to chemotaxis, including chemotropism, phototaxis, and electrotaxis
  • Details the application of cutting-edge technologies for stimulating cells with chemoattractants and analyzing their resulting cellular behaviors
  • Covers a variety of state-of-the-art microscopic methods for visualizing the migration of cells in tissues and the spatiotemporal dynamics of chemotaxis signaling molecules
  • Provides step-by-step functional assays for a variety of components involved in chemotaxis, including cell-surface receptors, downstream effectors, and cytoskeletal components
  • Explores the quantitative description of chemoattractant gradients and computational approaches for modeling the signaling networks that underlie gradient sensing
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  • ISBN 978-1-60761-198-1
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About this book

Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system.

Reviews

From the reviews: “The purpose is to provide an overview of and practical guidance on the diverse methodologies involved in chemotaxis research. … Each chapter includes an introduction to the topic, lists of necessary materials and reagents, laboratory protocol, and notes on troubleshooting and avoiding pitfalls, followed by current references. … This will serve as a valuable resource for students and scientists seeking practical guidance on diverse methodologies in chemotaxis research.” (Omer Iqbal, Doody’s Review Service, April, 2010)

Table of contents (33 chapters)

Table of contents (33 chapters)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-60761-198-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA in USD
Softcover $199.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Chemotaxis
Book Subtitle
Methods and Protocols
Editors
  • Tian Jin
  • Dale Hereld
Series Title
Methods in Molecular Biology
Series Volume
571
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-198-1
DOI
10.1007/978-1-60761-198-1
Hardcover ISBN
978-1-60761-197-4
Softcover ISBN
978-1-61779-642-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 538
Number of Illustrations
127 b/w illustrations, 5 illustrations in colour
Topics