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Zebrafish

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Graham J. Lieschke

    1. Walter & Eliza Hall Institute of, Medical Research, Royal Melbourne Hospital, Parkville, Australia

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  2. Andrew C. Oates

    1. und Genetik, MPI für Molekulare Zellbiologie, Dresden, Germany

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  3. Koichi Kawakami

    1. Div. Molecular & Developmental, Biology, National Institute of Genetics, Shizuoka, Japan

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  • Covers key techniques for zebrafish mutagenesis, transgenesis, and gene expression analysis and imaging
  • Contains practical approaches to chemical and insertional mutagenesis, including frozen storage of zebrafish sperm
  • Includes a range of transgenesis protocols (building transgenes from bacterial artificial chromosomes, transposon- and meganuclease-assisted transgenesis)
  • Describes imaging techniques ranging from organ-specific examples to whole embryo in toto time-lapse imaging
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 546)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mutagenesis and Mutants

    1. Front Matter

      Pages 1-1
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    2. Highly Efficient ENU Mutagenesis in Zebrafish

      • Ewart de Bruijn, Edwin Cuppen, Harma Feitsma
      Pages 3-12

    3. Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish

      • Li-En Jao, Shawn M. Burgess
      Pages 13-30

    4. Undertaking a Successful Gynogenetic Haploid Screen in Zebrafish

      • Judith E. Layton
      Pages 31-44

    5. Cryopreservation and In Vitro Fertilization at the Zebrafish International Resource Center

      • Carrie Carmichael, Monte Westerfield, Zoltán M. Varga
      Pages 45-65

  3. Transgenesis

    1. Front Matter

      Pages 68-68
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    2. Transient and Stable Transgenesis Using Tol2 Transposon Vectors

      • Hiroshi Kikuta, Koichi Kawakami
      Pages 69-84

    3. Analysis of Genes and Genome by the Tol2-Mediated Gene and Enhancer Trap Methods

      • Akihiro Urasaki, Koichi Kawakami
      Pages 85-102

    4. Bacterial Artificial Chromosome Transgenesis for Zebrafish

      • Zhongan Yang, Hong Jiang, Shuo Lin
      Pages 103-116

    5. Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish

      • Daniele Soroldoni, Benjamin M. Hogan, Andrew C. Oates
      Pages 117-130

  4. Tissue-Specific Manipulations

    1. Front Matter

      Pages 132-132
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    2. Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos

      • Harshan Pisharath, Michael J. Parsons
      Pages 133-143

    3. Focal Electroporation in Zebrafish Embryos and Larvae

      • Marcel Tawk, Isaac H. Bianco, Jonathan D. W. Clarke
      Pages 145-151

    4. Tissue Micromanipulation in Zebrafish Embryos

      • Alexander Picker, Daniela Roellig, Olivier Pourquié, Andrew C. Oates, Michael Brand
      Pages 153-172

  5. Analyzing Gene Expression

    1. Front Matter

      Pages 174-174
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    2. Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments. Part I: Array Printing and Hybridization

      • Siew Hong Lam, Sinnakarupan Mathavan, Zhiyuan Gong
      Pages 175-195

    3. Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and Analysis

      • Siew Hong Lam, R. Krishna Murthy Karuturi, Zhiyuan Gong
      Pages 197-226

    4. Validating microRNA Target Transcripts Using Zebrafish Assays

      • Luke Pase, Graham J. Lieschke
      Pages 227-240

  6. Imaging

    1. Front Matter

      Pages 242-242
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Keywords

About this book

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.

Editors and Affiliations

  • Walter & Eliza Hall Institute of, Medical Research, Royal Melbourne Hospital, Parkville, Australia

    Graham J. Lieschke

  • und Genetik, MPI für Molekulare Zellbiologie, Dresden, Germany

    Andrew C. Oates

  • Div. Molecular & Developmental, Biology, National Institute of Genetics, Shizuoka, Japan

    Koichi Kawakami

Bibliographic Information

  • Book Title: Zebrafish

  • Book Subtitle: Methods and Protocols

  • Editors: Graham J. Lieschke, Andrew C. Oates, Koichi Kawakami

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-977-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-976-5Published: 15 May 2009

  • Softcover ISBN: 978-1-62703-899-7Published: 27 November 2014

  • eBook ISBN: 978-1-60327-977-2Published: 20 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 335

  • Topics: Fish & Wildlife Biology & Management, Biochemistry, general, Animal Models

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