Methods in Molecular Biology

siRNA and miRNA Gene Silencing

From Bench to Bedside

Editors: Sioud, Mouldy (Ed.)

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  • Unparalleled diversity of siRNA delivery and imaging strategies covered in a single volume
  • Details the strategies that predict the design of effective siRNA with minimum side effects
  • Fully explains miRNA biogenesis and how to design microRNA-based vectors and apply them in vivo
  • Explores the clinical potential of siRNA in human diseases, and describes the first application in patients with cancer
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eBook $139.00
price for USA in USD
  • ISBN 978-1-60327-547-7
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $179.00
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About this book

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection.

Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Methods for Selecting Effective siRNA Sequences by using Statistical and Clustering Techniques

    Pages 1-39

    Takasaki , Shigeru

  • Deciphering the Code of Innate Immunity Recognition of siRNAs

    Pages 1-19

    Sioud , Mouldy

  • Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells

    Pages 1-22

    Sioud , Mouldy

  • Local and Systemic Delivery of siRNAs for Oligonucleotides Therapy

    Pages 1-10

    Takeshita , Fumitaka (et al.)

  • Imaging of siRNA Delivery and Silencing

    Pages 1-18

    Moore , Anna (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-60327-547-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
siRNA and miRNA Gene Silencing
Book Subtitle
From Bench to Bedside
Editors
  • Mouldy Sioud
Series Title
Methods in Molecular Biology
Series Volume
487
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-547-7
DOI
10.1007/978-1-60327-547-7
Hardcover ISBN
978-1-60327-546-0
Softcover ISBN
978-1-61737-908-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 472
Topics