Methods in Molecular Biology The Nucleus

The Nucleus

Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging

Editors: Hancock, Ronald (Ed.)

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  • Provides the latest protocols to study the mechanical properties of the nucleus and for imaging down to the molecular level by optical, atomic force, and electron microscopy

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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60327-461-6
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60327-460-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
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  • ISBN 978-1-61737-898-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging presents biophysical approaches to study the mechanical properties of nuclei, together with a comprehensive range of imaging methods. These include FISH, FRAP, FRET, molecular beacons, fluorescence correlation spectroscopy, single molecule tracking, and combing DNA for optical microscopy, recognition imaging for atomic force microscopy, and hybridisation, tomography, and spectroscopic imaging for electron microscopy.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

The Nucleus, Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging provides a comprehensive collection of the cutting-edge methods making a major contribution to understanding the nucleus and its nanostructure today.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Physical Properties of the Nucleus Studied by Micropipette Aspiration

    Pages 3-12

    Rowat, Amy C.

  • Mechanical Properties of Interphase Nuclei Probed by Cellular Strain Application

    Pages 13-26

    Lammerding, Jan (et al.)

  • Gene Expression in Polytene Nuclei

    Pages 29-54

    Björk, Petra (et al.)

  • Electron Microscope Visualization of RNA Transcription and Processing in Saccharomyces cerevisiae by Miller Chromatin Spreading

    Pages 55-69

    Osheim, Yvonne N. (et al.)

  • Combing Genomic DNA for Structural and Functional Studies

    Pages 71-90

    Schurra, Catherine (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60327-461-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60327-460-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61737-898-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Nucleus
Book Subtitle
Volume 2: Chromatin, Transcription, Envelope, Proteins, Dynamics, and Imaging
Editors
  • Ronald Hancock
Series Title
Methods in Molecular Biology
Series Volume
464
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: Atlantic Pub. & Distr. (P) Ltd., New Delhi, India
eBook ISBN
978-1-60327-461-6
DOI
10.1007/978-1-60327-461-6
Hardcover ISBN
978-1-60327-460-9
Softcover ISBN
978-1-61737-898-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 444
Number of Illustrations
82 b/w illustrations, 14 illustrations in colour
Topics