Overview
- Serves as a comprehensive guide to most of the current techniques for manipulating and analyzing C. difficile
- Written by scientists who have pioneered modern C. difficile research
- Brings together in one place chapters from clinicians, clinical laboratory scientist, and basic scientists to provide a comprehensive understanding of the practical state of the art in C. difficile research
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 646)
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Table of contents (15 protocols)
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Front Matter
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Introduction to Clostridium difficile and the Disease It Causes
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Front Matter
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Introduction to C. difficile and the Disease It Causes
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Isolation and Culture Techniques
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Front Matter
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Methods for Typing and Epidemiological Studies
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Front Matter
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Genomics
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Front Matter
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Development of Systems for Genetic Analysis of the Organism
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Front Matter
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Keywords
About this book
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.
Reviews
From the reviews:
“This book describes a variety of research methods used to investigate the pathogenic properties of Clostridium difficile, a major nosocomial pathogen that causes a severe diarrhea after prolonged antibiotic treatment. … This book would be useful to scientists working with bacteria such as C. difficile that cause toxin-mediated diseases in the colon of their host. … This is a technical book that provides essential methods and techniques to study devastating diseases due to Clostridium difficile.” (Rebecca T. Horvat, Doody’s Review Service, October, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Clostridium difficile
Book Subtitle: Methods and Protocols
Editors: Peter Mullany, Adam P. Roberts
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-365-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60327-364-0Published: 08 June 2010
Softcover ISBN: 978-1-4939-5701-9Published: 23 August 2016
eBook ISBN: 978-1-60327-365-7Published: 03 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 241
Topics: Microbiology, Infectious Diseases, Immunology, Pathology, Laboratory Medicine