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Histology Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Tim D. Hewitson

    1. Dept. Nephrology, Royal Hospital Melbourne, Melbourne, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Ian A. Darby

    1. School of Medical Sciences, Cancer & Tissue Repair Research Group, RMIT University, Melbourne, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides an easily accessible reference volume for histological techniques written by leading researchers in the field
  • Details the techniques for investigating the expression of many different types of targets in a variety of tissue and samples from sections and cells to electron microscopy
  • Covers a wide range of techniques from classical histochemistry to detection of mRNA, apoptosis, and hypoxia
  • Step-by-step guide to techniques including extensive notes to aid in troubleshooting techniques
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 611)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-viii
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  3. Staining Techniques

    1. Front Matter

      Pages 41-41
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    2. Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells

      • Josiane Smith-Clerc, Boris Hinz
      Pages 43-57

    3. Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems

      • Vincent Sarrazy, Alexis Desmoulière
      Pages 59-71

    4. Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections

      • Emilio Badoer
      Pages 73-85

    5. High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy

      • Christian Mühlfeld
      Pages 87-101

    6. Lectin Histochemistry for Light and Electron Microscopy

      • Su Ee Wong, Catherine E. Winbanks, Chrishan S. Samuel, Tim D. Hewitson
      Pages 103-114

    7. Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain

      • Raphael Pinaud, Jin K. Jeong
      Pages 115-129

    8. Special Stains for Extracellular Matrix

      • Andréa Monte-Alto-Costa, Luís Cristóvão Porto
      Pages 131-140

    9. Active Staining of Mouse Embryos for Magnetic Resonance Microscopy

      • Alexandra Petiet, G. Allan Johnson
      Pages 141-149

    10. Immunohistochemical Detection of Tumour Hypoxia

      • Richard J. Young, Andreas Möller
      Pages 151-159

    11. In Situ Localization of Apoptosis Using TUNEL

      • Tim D. Hewitson, Ian A. Darby
      Pages 161-170

  4. Imaging Techniques

    1. Front Matter

      Pages 171-171
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    2. Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord

      • Martin Stebbing, Simon Potocnik, Pinglu Ye, Emilio Badoer
      Pages 173-182

    3. High-Resolution Confocal Imaging in Tissue

      • Verena C. Wimmer, Andreas Möller
      Pages 183-191

    4. Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography

      • Jörg Piper
      Pages 193-210
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Keywords

About this book

Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.

Reviews

From the reviews:

“This slim volume aims to provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques to realise their potential of their resource. … I enjoyed reading this book … . this book is not particularly expensive and is so comprehensive and well written that I would certainly recommend it to anyone thinking of setting up a research project but uncertain what sort of method to use. It would also be a valuable asset to any departmental library … .” (Anne Patricia Campbell, Bulletin of the Royal Society of Pathologists, Issue 154, April, 2011)

“Reports the basic techniques of immunohistochemistry and molecular biology used to optimize resources and obtain accurate results. … This book is recommended to students and researchers using the laboratory techniques on histology described. Updated protocols are described by renowned scientists in the area using understandable language while details of possible interference are given for each technique. Tim D. Hewiston and Ian A. Darby have undertaken several studies on histology, many of which were conducted in collaboration with the authors contributing to this book.” (Rui Curi, Brazilian Journal of Pharmaceutical Sciences, Spring, 2011)

“This volume in the Methods in Molecular Biology series provides a compendium of proven protocols for modern histology. … Written for basic science and clinical researchers interested in mapping the location and distribution of biomarkers, the book also will be appreciated by lecturers and students specializing in biomedical technology, histology, and clinical laboratory medicine. … The book is well written and carefully edited, and will be a welcome resource for all basic science and clinical researchers studying modern cell biology.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

Editors and Affiliations

  • Dept. Nephrology, Royal Hospital Melbourne, Melbourne, Australia

    Tim D. Hewitson

  • School of Medical Sciences, Cancer & Tissue Repair Research Group, RMIT University, Melbourne, Australia

    Ian A. Darby

Bibliographic Information

  • Book Title: Histology Protocols

  • Editors: Tim D. Hewitson, Ian A. Darby

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-345-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2010

  • Hardcover ISBN: 978-1-60327-344-2Published: 16 December 2009

  • Softcover ISBN: 978-1-4939-5694-4Published: 23 August 2016

  • eBook ISBN: 978-1-60327-345-9Published: 04 December 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 229

  • Topics: Cell Biology, Biochemistry, general, Laboratory Medicine

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