Methods in Molecular Biology

Histology Protocols

Editors: Hewitson, Tim D., Darby, Ian A. (Eds.)

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  • Provides an easily accessible reference volume for histological techniques written by leading researchers in the field
  • Details the techniques for investigating the expression of many different types of targets in a variety of tissue and samples from sections and cells to electron microscopy
  • Covers a wide range of techniques from classical histochemistry to detection of mRNA, apoptosis, and hypoxia
  • Step-by-step guide to techniques including extensive notes to aid in troubleshooting techniques
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eBook $109.00
price for USA in USD
  • ISBN 978-1-60327-345-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
Softcover $149.00
price for USA in USD
About this book

With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field contribute detailed methods involving the preparation of tissue, providing an overview of fixation, embedding, and processing, along with the required techniques for the retrieval of RNA from histological sections, followed by sections on routine and specialist histological staining techniques, including immuno-, lectin, and hybridization histochemistry, histological staining, as well as specific methods for the in situ identification of hypoxia and apoptosis, and details on the advances in imaging and image analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Histology Protocols will provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource.

Reviews

From the reviews:

“This slim volume aims to provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques to realise their potential of their resource. … I enjoyed reading this book … . this book is not particularly expensive and is so comprehensive and well written that I would certainly recommend it to anyone thinking of setting up a research project but uncertain what sort of method to use. It would also be a valuable asset to any departmental library … .” (Anne Patricia Campbell, Bulletin of the Royal Society of Pathologists, Issue 154, April, 2011)

“Reports the basic techniques of immunohistochemistry and molecular biology used to optimize resources and obtain accurate results. … This book is recommended to students and researchers using the laboratory techniques on histology described. Updated protocols are described by renowned scientists in the area using understandable language while details of possible interference are given for each technique. Tim D. Hewiston and Ian A. Darby have undertaken several studies on histology, many of which were conducted in collaboration with the authors contributing to this book.” (Rui Curi, Brazilian Journal of Pharmaceutical Sciences, Spring, 2011)

“This volume in the Methods in Molecular Biology series provides a compendium of proven protocols for modern histology. … Written for basic science and clinical researchers interested in mapping the location and distribution of biomarkers, the book also will be appreciated by lecturers and students specializing in biomedical technology, histology, and clinical laboratory medicine. … The book is well written and carefully edited, and will be a welcome resource for all basic science and clinical researchers studying modern cell biology.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)


Table of contents (17 chapters)

Table of contents (17 chapters)
  • Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy

    Pages 3-18

    Hewitson, Tim D. (et al.)

  • An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue

    Pages 19-27

    Chung, Joon-Yong (et al.)

  • Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus

    Pages 29-40

    Edgley, Amanda J. (et al.)

  • Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells

    Pages 43-57

    Smith-Clerc, Josiane (et al.)

  • Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems

    Pages 59-71

    Sarrazy, Vincent (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-60327-345-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
Softcover $149.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Histology Protocols
Editors
  • Tim D. Hewitson
  • Ian A. Darby
Series Title
Methods in Molecular Biology
Series Volume
611
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-345-9
DOI
10.1007/978-1-60327-345-9
Hardcover ISBN
978-1-60327-344-2
Softcover ISBN
978-1-4939-5694-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 229
Topics