Methods in Molecular Biology

Cell-Free Protein Production

Methods and Protocols

Editors: Endo, Yaeta, Takai, Kazuyuki, Ueda, Takuya (Eds.)

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  • Covers cell-free expression systems including E. coli, wheat, insect and human cells as well as a totally reconstituted system
  • Shows how cell-free expression can be applied to high-throughput and one-by-one protein functional and structural analyses
  • Provides rapid methods for protein engineering and the creation of new proteins
  • Presents basic methodologies that can support constitutive approaches for understanding complex molecular systems
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-60327-331-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-60327-330-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5698-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

Table of contents (20 chapters)

  • Cell-Free Protein Preparation Through Prokaryotic Transcription–Translation Methods

    Kigawa, Takanori

    Pages 1-10

  • PURE Technology

    Shimizu, Yoshihiro (et al.)

    Pages 11-21

  • The Cell-Free Protein Synthesis System from Wheat Germ

    Takai, Kazuyuki (et al.)

    Pages 23-30

  • A Cell-Free Protein Synthesis System from Insect Cells

    Ezure, Toru (et al.)

    Pages 31-42

  • Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells

    Mikami, Satoshi (et al.)

    Pages 43-52

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-60327-331-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-60327-330-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-4939-5698-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell-Free Protein Production
Book Subtitle
Methods and Protocols
Editors
  • Yaeta Endo
  • Kazuyuki Takai
  • Takuya Ueda
Series Title
Methods in Molecular Biology
Series Volume
607
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-331-2
DOI
10.1007/978-1-60327-331-2
Hardcover ISBN
978-1-60327-330-5
Softcover ISBN
978-1-4939-5698-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 261
Topics