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Methods in Molecular Biology

Bioluminescence

Methods and Protocols

Editors: Rich, Preston B., Douillet, Christelle (Eds.)

  • Written by leading researchers in the field
  • Provides an accessible reference for a wide range of bioluminescence assays and applications
  • Serves as a comprehensive guide to developing, validating, running, and analyzing bioluminescence assays
  • Develops detection and imaging methods
  • Describes the use of a wide variety of bioluminescence tools
  • Presents protocols with enough details to be followed and adapted by scientific teams with no previous experience in bioluminescence
  • Contains numerous notes about potential pitfalls and tips on how to avoid failures
  • Presents cutting-edge material that will also interest experts in the field of bioluminescence
  • Provides methods and assays that can be adapted and used in most specialties of biomedical and biotechnological fields
  • Covers a wide range of sub-cellular and cell-based assays for in vitro and in vivo analysis as well as non invasive, in vivo, and real time assays and monitoring for animal models
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60327-321-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60327-320-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61779-752-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Through the study and application of bioluminescence, scientists have painstakingly harnessed a powerful tool that enables us to seek a deeper understanding of the complex mechanisms underpinning so many vital biologic systems. In this fully revised and updated second edition of Bioluminescence: Methods and Protocols, expert researchers contribute a readable and utilitarian compilation of the newest and most innovative techniques that have emerged in this rapidly expanding and progressively diverse field including methods to assess cell trafficking, protein-protein interactions, intracellular signaling, and apoptosis. Also opening up the possibility to visualize and quantify biological mechanisms in real-time and in in vivo settings, the volume also describes the in vivo study of bacterial or viral infections, transplanted cells, stem cells proliferation, vascular flow, and tumors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials, equipment, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescence: Methods and Protocols, Second Edition provides protocols that are detailed enough to be followed and adapted by scientific teams who have no previous expertise in bioluminescence in order to foster the potentially numerous breakthroughs and new applications from basic to applied science and medicine that must continue to be developed.

Reviews

From the reviews of the second edition: “This is a compilation of laboratory protocols dealing with diverse applications of bioluminescence in the biological sciences. … The book is written primarily for scientists working in the field of biomedical sciences. … It also provides a number of updated protocols and examples for practicing investigators interested in using bioluminescence in their research. … This second edition should prove to be a valuable companion to all researchers interested in bioluminescence.” (Ruben Mestril, Doody’s Review Service, December, 2009)

Table of contents (20 chapters)

  • Luminescent Probes and Visualization of Bioluminescence

    Michelini, Elisa (et al.)

    Pages 1-13

  • Validation of Bioluminescent Imaging Techniques

    Virostko, John (et al.)

    Pages 15-23

  • Assessment of Extracellular ATP Concentrations

    Seminario-Vidal, Lucia (et al.)

    Pages 25-36

  • High-Throughput Quantitative Bioluminescence Imaging for Assessing Tumor Burden

    Contero, Angelina (et al.)

    Pages 37-45

  • Fluorescence Imaging of Tumors with “Smart” pH-Activatable Targeted Probes

    Asanuma, Daisuke (et al.)

    Pages 47-62

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-60327-321-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-60327-320-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61779-752-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bioluminescence
Book Subtitle
Methods and Protocols
Editors
  • Preston B. Rich
  • Christelle Douillet
Series Title
Methods in Molecular Biology
Series Volume
574
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-321-3
DOI
10.1007/978-1-60327-321-3
Hardcover ISBN
978-1-60327-320-6
Softcover ISBN
978-1-61779-752-1
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 268
Number of Illustrations and Tables
31 b/w illustrations, 29 illustrations in colour
Topics