Methods in Molecular Biology

Membrane Proteomics

Methods and Protocols

Editors: Peirce, Matthew J., Wait, Robin (Eds.)

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  • Collects together in one volume the diverse practical approaches to analyzing membrane proteins
  • Includes both wet lab and in silico approaches
  • Includes protocols for the isolation of plasma membrane and organellar membrane proteins
  • Covers both prokaryotic and eukaryotic membrane proteins
  • Describes methods useful and accessible to both novice and dedicated proteomic laboratories
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eBook $139.00
price for USA in USD
  • ISBN 978-1-60327-310-7
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase and usable on all devices
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Hardcover $179.99
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Softcover $179.00
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About this book

With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compile a laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, in silico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and easy-to-use, Membrane Proteomics: Methods and Protocols is an ideal guide for investigators wishing to apply state of the art membrane proteomic methodologies in their own research programs.

Reviews

From the reviews:

"This is an important resource for investigators interested in understanding patterns of membrane protein expression … which are helpful in the identification of novel markers of, or targets for intervention in, disease. … It will be quite useful for research investigators and students interested in this field. … This book will be a valuable resource for investigators and students interested in learning about membrane proteomic methodologies. … High quality illustrations greatly help the understanding of the material." (Omer Iqbal, Doody’s Review Service, June, 2009)


Table of contents (23 chapters)

Table of contents (23 chapters)
  • Online Tools for Predicting Integral Membrane Proteins

    Pages 3-23

    Bigelow, Henry (et al.)

  • In Silico Identification of Novel G Protein Coupled Receptors

    Pages 25-36

    Davies, Matthew N. (et al.)

  • Transcriptome-Based Identification of Candidate Membrane Proteins

    Pages 37-56

    Evans, Edward J. (et al.)

  • Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis

    Pages 60-70

    D’Amici, Gian Maria (et al.)

  • Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning

    Pages 72-81

    Everberg, Henrik (et al.)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-60327-310-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.99
price for USA in USD
Softcover $179.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Membrane Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Matthew J. Peirce
  • Robin Wait
Series Title
Methods in Molecular Biology
Series Volume
528
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-310-7
DOI
10.1007/978-1-60327-310-7
Hardcover ISBN
978-1-60327-309-1
Softcover ISBN
978-1-61737-889-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 342
Topics