Methods in Molecular Biology

Therapeutic Applications of RNAi

Methods and Protocols

Editors: Reidhaar-Olson, John F., Rondinone, Cristina M. (Eds.)

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  • Provides detailed protocols for numerous methods related to in vivo use of RNAi
  • Describes a variety of delivery methods and model systems for studying therapeutic applications of RNAi
  • Covers RNAi applications in oncology, infectious disease and CNS disorders
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eBook $119.00
price for USA in USD
  • ISBN 978-1-60327-295-7
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Hardcover $159.99
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Softcover $159.99
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About this book

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.

Reviews

From the reviews: “This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized in 10 chapters dealing with the … task of delivering RNA interference molecules (RNAi) to live organisms to achieve therapeutic effects. … The book is written mainly for scientists, clinical researchers, and all investigators interested in the field of biotherapeutic research.” (Ruben Mestril, Doody’s Review Service, January, 2010)

Table of contents (10 chapters)

Table of contents (10 chapters)
  • Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors

    Pages 1-13

    Medarova, Zdravka (et al.)

  • Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery

    Pages 15-27

    Golzio, Muriel (et al.)

  • Liposomal siRNA for Ovarian Cancer

    Pages 29-42

    Mangala, Lingegowda S. (et al.)

  • Therapeutic Applications of RNAi for Silencing Virus Replication

    Pages 43-61

    Tripp, Ralph A. (et al.)

  • RNAi in the Malaria Vector, Anopheles gambiae

    Pages 63-75

    Catteruccia, Flaminia (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-60327-295-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
  • Institutional customers should get in touch with their account manager
Hardcover $159.99
price for USA in USD
Softcover $159.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Therapeutic Applications of RNAi
Book Subtitle
Methods and Protocols
Editors
  • John F. Reidhaar-Olson
  • Cristina M. Rondinone
Series Title
Methods in Molecular Biology
Series Volume
555
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-295-7
DOI
10.1007/978-1-60327-295-7
Hardcover ISBN
978-1-60327-294-0
Softcover ISBN
978-1-61779-494-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 148
Number of Illustrations
12 b/w illustrations, 6 illustrations in colour
Topics