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Therapeutic Applications of RNAi

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Cristina M. Rondinone
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    You can also search for this editor in PubMed   Google Scholar

  2. John F. Reidhaar-Olson
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  • Provides detailed protocols for numerous methods related to in vivo use of RNAi
  • Describes a variety of delivery methods and model systems for studying therapeutic applications of RNAi
  • Covers RNAi applications in oncology, infectious disease and CNS disorders

Part of the book series: Methods in Molecular Biology (MIMB, volume 555)

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Table of contents (10 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Development and Application of a Dual-Purpose Nanoparticle Platform for Delivery and Imaging of siRNA in Tumors

    • Zdravka Medarova, Mohanraja Kumar, Shu-wing Ng, Anna Moore
    Pages 1-13

  3. Targeted Gene Silencing into Solid Tumors with Electrically Mediated siRNA Delivery

    • Muriel Golzio, Laurent Mazzolini, Aurélie Paganin-Gioanni, Justin Teissié
    Pages 15-27

  4. Liposomal siRNA for Ovarian Cancer

    • Lingegowda S. Mangala, Hee Dong Han, Gabriel Lopez-Berestein, Anil K. Sood
    Pages 29-42

  5. Therapeutic Applications of RNAi for Silencing Virus Replication

    • Ralph A. Tripp, Stephen Mark Tompkins
    Pages 43-61

  6. RNAi in the Malaria Vector, Anopheles gambiae

    • Flaminia Catteruccia, Elena A. Levashina
    Pages 63-75

  7. RNAi Using a Chitosan/siRNA Nanoparticle System: In Vitro and In Vivo Applications

    • Morten Østergaard Andersen, Kenneth Alan Howard, Jørgen Kjems
    Pages 77-86

  8. Lentiviral and Adeno-Associated Vector-Based Therapy for Motor Neuron Disease Through RNAi

    • Chris Towne, Patrick Aebischer
    Pages 87-108

  9. Studying Autoimmunity by In Vivo RNA Interference

    • Stephan Kissler
    Pages 109-118

  10. Transcriptional Gene Silencing Using Small RNAs

    • Daniel H. Kim, John J. Rossi
    Pages 119-125

  11. Alternative Splicing as a Therapeutic Target for Human Diseases

    • Kenneth J. Dery, Veronica Gusti, Shikha Gaur, John E. Shively, Yun Yen, Rajesh K. Gaur
    Pages 127-144

  12. Back Matter

    Pages 145-148
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Keywords

About this book

The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challenges remain, particulary in the area of delivery. Therapeutic Applications of RNAi provides detailed protocols in key areas of current focus, including testing of delivery vehicles, identification of appropriate model systems, and evaluation of the effects of RNAi in vivo. Produced by a team of internationally renowned authors, the volume describes the therapeutic applications of RNAi and potential pitfalls in oncology, viral infections and CNS disease, using a variety of delivery methods, including liposomes, peptide-based nanoparticles, polycationic polymers, and viral vehicles. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Therapeutic Applications of RNAi is an ideal guide for scientists attempting to solve the numerous challenges in this field and revolutionize the treatment of disease.

Reviews

From the reviews: “This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized in 10 chapters dealing with the … task of delivering RNA interference molecules (RNAi) to live organisms to achieve therapeutic effects. … The book is written mainly for scientists, clinical researchers, and all investigators interested in the field of biotherapeutic research.” (Ruben Mestril, Doody’s Review Service, January, 2010)

Bibliographic Information

  • Book Title: Therapeutic Applications of RNAi

  • Book Subtitle: Methods and Protocols

  • Editors: Cristina M. Rondinone, John F. Reidhaar-Olson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-295-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-294-0Published: 09 July 2009

  • Softcover ISBN: 978-1-61779-494-0Published: 01 December 2011

  • eBook ISBN: 978-1-60327-295-7Published: 04 June 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 148

  • Number of Illustrations: 12 b/w illustrations, 6 illustrations in colour

  • Topics: Pharmacology/Toxicology, Biochemical Engineering

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