Methods in Molecular Biology

Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Editors: Jain, S. Mohan, Saxena, Praveen (Eds.)

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  • Provides step-by-step protocols for the establishment of in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment
  • Provides step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60327-287-2
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  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60327-286-5
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-886-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Reviews

From the reviews:

“This technical text offers step-by-step protocols for the laboratory technician as part of the Methods in Molecular biology series. It shows research that aids in the conservation of medical herbs, especially those species that have been identified as being environmentally at risk. The book’s other goal is to improve the quality and consistency of herbal products through chemical analysis, cloning, cultures, and secondary metabolites.” (American Herb Association Quarterly, Vol. 25 (4), 2011)

“This book … covers a range of topics vital for the continued production of this essential but sometimes forgotten group of plants. … The chapters are easy to read and the protocols are easy to follow. All the necessary information is within the chapter. … In conclusion, I would recommend this book to researchers and students working in the area of plant tissue culture and biochemical analysis. This book would also be beneficial as a text book or laboratory manual in plant tissue culture courses.” (Alison M. R. Ferrie, Annals of Botany, Vol. 105 (4), April, 2010)

“This technical guide for plant research helps explains the processes Step by-step procedures describemethods of analyzing plant compounds, identify molecular markers to find them … . It includes materials researchers need and common pitfalls they can avoid. … It is also designed to help conserve germ plasma and cryopreservation of medicinal plant species that face extinction or adverse effects from climate change.” (The American Herb Association, Vol. 25 (2), 2010)


Table of contents (32 chapters)

Table of contents (32 chapters)
  • Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives

    Pages 3-16

    Paek, Kee-Yoeup (et al.)

  • In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantitation of Reserpine, Ajmaline, and Ajmalicine

    Pages 17-33

    Goel, M. K. (et al.)

  • Medicinal Properties, In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine

    Pages 35-52

    Häggman, Hely (et al.)

  • Saussurea medusa Cell Suspension Cultures for Flavonoid Production

    Pages 53-59

    Liu, Chun-Zhao (et al.)

  • Large-scale In Vitro Multiplication of Crataeva nurvala

    Pages 61-70

    Babbar, Shashi B. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-60327-287-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-60327-286-5
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-886-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants
Editors
  • S. Mohan Jain
  • Praveen Saxena
Series Title
Methods in Molecular Biology
Series Volume
547
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-60327-287-2
DOI
10.1007/978-1-60327-287-2
Hardcover ISBN
978-1-60327-286-5
Softcover ISBN
978-1-61737-886-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 411
Number of Illustrations
113 b/w illustrations
Topics