Overview
- Provides step-by-step protocols for the establishment of in vitro cultures of key medicinal plants and their mass multiplication in a controlled environment
- Provide step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 547)
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Table of contents (32 protocols)
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Front Matter
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Micropropagation and Chemical Analysis
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Front Matter
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Transgenic Approaches
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Front Matter
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Keywords
About this book
Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, expert researchers provide detailed, step-by-step protocols for the establishment of in vitro cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of in vitro germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.
Reviews
From the reviews:
“This technical text offers step-by-step protocols for the laboratory technician as part of the Methods in Molecular biology series. It shows research that aids in the conservation of medical herbs, especially those species that have been identified as being environmentally at risk. The book’s other goal is to improve the quality and consistency of herbal products through chemical analysis, cloning, cultures, and secondary metabolites.” (American Herb Association Quarterly, Vol. 25 (4), 2011)
“This book … covers a range of topics vital for the continued production of this essential but sometimes forgotten group of plants. … The chapters are easy to read and the protocols are easy to follow. All the necessary information is within the chapter. … In conclusion, I would recommend this book to researchers and students working in the area of plant tissue culture and biochemical analysis. This book would also be beneficial as a text book or laboratory manual in plant tissue culture courses.” (Alison M. R. Ferrie, Annals of Botany, Vol. 105 (4), April, 2010)
“This technical guide for plant research helps explains the processes Step by-step procedures describemethods of analyzing plant compounds, identify molecular markers to find them … . It includes materials researchers need and common pitfalls they can avoid. … It is also designed to help conserve germ plasma and cryopreservation of medicinal plant species that face extinction or adverse effects from climate change.” (The American Herb Association, Vol. 25 (2), 2010)
Editors and Affiliations
Bibliographic Information
Book Title: Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants
Editors: S. Mohan Jain, Praveen K. Saxena
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-287-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-286-5Due: 29 May 2009
Softcover ISBN: 978-1-61737-886-7Published: 19 November 2010
eBook ISBN: 978-1-60327-287-2Published: 12 June 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 411
Number of Illustrations: 113 b/w illustrations
Topics: Plant Genetics and Genomics, Biochemistry, general, Pharmacology/Toxicology, Plant Biochemistry, Cell Culture