Happy holidays from us to you—get up to $30 off your next print or eBook! Shop now >>

Methods in Molecular Biology

Affinity Chromatography

Methods and Protocols

Editors: Bailon, Pascal (Ed.)

Buy this book

eBook $54.99
price for USA in USD (gross)
  • ISBN 978-1-60327-261-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
About this book

Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.

Table of contents (19 chapters)

  • An Overview of Affinity Chromatography

    Wilchek, Meir (et al.)

    Pages 1-6

  • Weak Affinity Chromatography

    Strandh, Magnus (et al.)

    Pages 7-23

  • Fluidized-Bed Receptor-Affinity Chromatography

    Spence, Cheryl L. (et al.)

    Pages 25-39

  • Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports

    Podlaski, Frank J. (et al.)

    Pages 41-48

  • Affinity Purification of Monoclonal Antibodies

    Schwarz, Alexander

    Pages 49-56

Buy this book

eBook $54.99
price for USA in USD (gross)
  • ISBN 978-1-60327-261-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Affinity Chromatography
Book Subtitle
Methods and Protocols
Editors
  • Pascal Bailon
Series Title
Methods in Molecular Biology
Series Volume
147
Copyright
2000
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-60327-261-2
DOI
10.1007/978-1-60327-261-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 230
Number of Illustrations
39 b/w illustrations
Topics