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Methods in Molecular Biology

Inositol Phosphates and Lipids

Methods and Protocols

Editors: Barker, Christopher J. (Ed.)

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  • -Serves as an easily accessible reference volume for all aspects of measurement of inositol phosphates and lipids contributed by front-line researchers in the field
  • -Combines in one volume methodologies for both inositol phosphates and lipids and the key enzymes which interconnect them
  • -Covers the new field of inositol pyrophosphates as well as several established areas in the lipid and phosphate arena
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eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-60327-175-2
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Hardcover $159.99
price for USA in USD
  • ISBN 978-1-60327-174-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-1-61779-689-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.

Table of contents (14 chapters)

Table of contents (14 chapters)
  • The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells

    Pages 1-19

    Barker, Christopher J. (et al.)

  • HPLC Separation of Inositol Polyphosphates

    Pages 21-46

    Barker, Christopher J. (et al.)

  • Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells

    Pages 47-60

    Otto, James C. (et al.)

  • A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate

    Pages 61-71

    Letcher, Andrew J. (et al.)

  • Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1)

    Pages 73-85

    Azevedo, Cristina (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-60327-175-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-60327-174-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-1-61779-689-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Inositol Phosphates and Lipids
Book Subtitle
Methods and Protocols
Editors
  • Christopher J. Barker
Series Title
Methods in Molecular Biology
Series Volume
645
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-175-2
DOI
10.1007/978-1-60327-175-2
Hardcover ISBN
978-1-60327-174-5
Softcover ISBN
978-1-61779-689-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 230
Number of Illustrations
54 b/w illustrations
Topics