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Methods in Molecular Biology

Laser Capture Microdissection

Methods and Protocols

Editors: Murray, Graeme I., Curran, Stephanie (Eds.)

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About this book

Use of laser microdissection has grown enormously to become a very powerful high-throughput technology essential to a range of downstream applications in pathology, cancer research, life sciences, medical diagnostics, and biotechnology. In Laser Capture Microdissection, expert laboratory researchers noted for their optimized methods describe in step-by-step detail not only how to successfully carry out tissue-based laser microdissection in their own laboratories using the different systems available, but also how to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. These readily reproducible methods make it possible to carry out contamination-free molecular analyses of specific types of normal and diseased cells. Highlights include gene expression profiling of primary tumor cell populations, RNA transcript amplification and GeneChip® microarrays, colorectal cancer proteomics, and proteomic analysis of human bladder tissue using the SELDI® approach. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Laser Capture Microdissection provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems and to the interpretation of the results using a wide range of sophisticated molecular technologies.

Reviews

From the reviews:

"...an excellent help to researchers...." - Neoplasma

"Comprehensive and state of the art, this book provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems, and also a guide to interpreting the results using a wide range of sophisticated molecular technologies." - Clinical Laboratory International

"...provides researchers with practical advise and detailed technical protocols for carrying out tissue-based LCM...." - Vet Pathol

"Comprehensive and up-to-date collection of readily reproducible preparative and analytical methods is presented in each chapter for an in-depth practice of laser microdissection application. … The book is subdivided into 5 parts with a total of 24 chapters featuring step-by-step protocols that will enable novice researchers to isolate specific cell types or microorganisms." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)


Table of contents (24 chapters)

Table of contents (24 chapters)
  • An Introduction to Laser-Based Tissue Microdissection Techniques

    Pages 3-7

    Curran, Stephanie (et al.)

  • Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells

    Pages 11-26

    Shibutani, Makoto (et al.)

  • Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue

    Pages 27-38

    Patrick, H.

  • comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue

    Pages 40-56

    Callagy, Grace (et al.)

  • Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP

    Pages 57-67

    Dillon, Deborah (et al.)

Buy this book

eBook $99.00
price for USA in USD
  • ISBN 978-1-59259-853-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Laser Capture Microdissection
Book Subtitle
Methods and Protocols
Editors
  • Graeme I. Murray
  • Stephanie Curran
Series Title
Methods in Molecular Biology
Series Volume
293
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-853-3
DOI
10.1385/1592598536
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 320
Number of Illustrations
81 b/w illustrations, 1 illustrations in colour
Topics

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