Pain Research

1. Front Matter

   Pages i-xi

   PDF

2. Mechanistic Dissection of Pain

   • Z. David Luo

   Pages 1-10

3. Assessment of Acute Thermal Nociception in Laboratory Animals

   • Jeffrey W. Allen, Tony L. Yaksh

   Pages 11-23

4. Tissue Injury Models of Persistent Nociception in Rats

   • Jeffrey W. Allen, Tony L. Yaksh

   Pages 25-34

5. Segmental Spinal Nerve Ligation Model of Neuropathic Pain

   • Jin Mo Chung, Hee Kee Kim, Kyungsoon Chung

   Pages 35-45

6. Partial Sciatic Nerve Transection

   • Thies Lindenlaub, Claudia Sommer

   Pages 47-53

7. Modeling Diabetic Sensory Neuropathy in Rats

   • Nigel A. Calcutt

   Pages 55-65

8. Sciatic Inflammatory Neuropathy in the Rat

   • Erin D. Milligan, Steven F. Maier, Linda R. Watkins

   Pages 67-89

9. A Rat Pain Model of Vincristine-Induced Neuropathy

   • Emiliano S. Higuera, Z. David Luo

   Pages 91-98

10. Loop Dialysis Catheter

    • Martin Marsala

    Pages 99-108

11. Intrathecal Catheterization and Drug Delivery in the Rat

    • Shelle A. Malkmus, Tony L. Yaksh

    Pages 109-121

12. Trigeminal Neuronal Recording in Animal Models of Orofacial Pain

    • Koichi Iwata, Yuji Masuda, Ke Ren

    Pages 123-137

13. In Vivo Electrophysiology of Dorsal-Horn Neurons

    • Louise C. Stanfa, Anthony H. Dickenson

    Pages 139-153

14. Single-Fiber Recording

    • Maria Schäfers, David Cain

    Pages 155-166

15. Anatomical Identification of Neurons Responsive to Nociceptive Stimuli

    • Luc Jasmin, Peter T. Ohara

    Pages 167-188

16. Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats

    • Thomas H. Burkey, Cynthia M. Hingtgen, Michael R. Vasko

    Pages 189-202

17. Primary Cultures of Neonatal Rat Spinal Cord

    • Virginia S. Seybold, Lia G. Abrahams

    Pages 203-213

18. Single-Cell Laser-Capture Microdissection and RNA Amplification

    • Fredrik Kamme, Jessica Zhu, Lin Luo, Jingxue Yu, Da-Thao Tran, Bernhard Meurers et al.

    Pages 215-223

19. Semi-Quantitative Real-Time PCR for Pain Research

    • Hong Qing Guo, Sandra R. Chaplan

    Pages 225-238

20. Functional Genomic Analysis in Pain Research Using Hybridization Arrays

    • Stephen J. Walker, Travis J. Worst, Willard M. Freeman, Kent E. Vrana

    Pages 239-253

The detrimental impacts of pain on the quality of our daily life have drawn increasing attention from researchers, health care providers, policymakers, and social workers. The reality of effective painkillers specifically designed for different types of pain states has been obscured by missing knowledge of the mechanisms of different types of pain. Thus, studying the complexity of pain transduction, which includes various insults to the peripheral nervous systems, sensitized spinal circuits, and altered signals ascending to or descending from the brain, has emerged as a high priority task on the agenda of pharmaceutical companies and other private as well as public agencies. To accomplish this mission, one requires a combination of well-integrated systems, such as a- mal models resembling the pathological conditions of pain transduction, and an understanding of the interactions among pain transducers and mediators at the molecular level. Thanks to rapid advancements in the developmentof novel cellular and molecular biology techniques, as well as in our understanding of physiology, and of the behavioral pharmacology of pain transduction, the time is now ripe for dissecting the molecular mechanisms of pain transduction using multidisciplinary approaches. Indeed, my acceptance of the invitation from the series editor, Dr. John Walker, to assemble a book of methods and protocols for pain research was inspired by these emerging needs. The purpose of Pain Research: Methods and Protocols is to provide st- by-step methods and protocols of multidisciplinary approaches related to the study of pain transduction.

• Department of Anesthesiology, University of California Irvine Medical Center, Irvine

  Z. David Luo

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