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- About this book
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In RNA-Protein Interaction Protocols, highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized, techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells. There are also in vitro and in vivo methods and protocols for assaying the effects of proteins and RNP complexes on mRNA metabolism. The methods assume only a knowledge of basic molecular biology, biochemistry, and cell culture, and can be readily adapted to other systems.
Providing an excellent standard resource for today's bench scientists studying many types of RNA-protein interactions, RNA-Protein Interaction Protocols offers a wide-ranging collection of up-to-date experimental methods that distill a great deal of wisdom and experience into productive, reproducible, step-by-step techniques for all investigators in the field. - Reviews
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"This manual is recommended to investigators with experience in molecular biological research, wishing to become introduced into the field of RNA-protein interactions. This book will also be a valuable source of reference for scientists needing general information about the theoretical basis and current methodological approaches to investigating ribonucleoprotein complexes."-International Journal of Medical Microbiology
"The coverage of the book is broad and reasonably comprehensive, with chapters arranged in a coherent sequence....Many of the authors are well-known, leading researchers in their field, and describe key contemporary techniques. In these respects the coverage is authoritative and likely to remain relevant for some years, before being overtaken by new technologies and methods...represents value for money."-Cell Biology International
"This is a book which any scientist involved in any kind of cellular and molecular biology will find profitable for his experimental investigations since RNA and proteins are unavoidable matters to solve any problem in Life Sciences. Highly skilled experimentalists provide a comprehensive series of commonly used, as well as specialized techniques for analyzing how proteins and RNA interact. Richly detailed and readily reproducible, these methods enable researchers to analyze the structural details of an RNA-protein interaction, to determine in detail what parts of the protein and RNA are in close contact, and to isolate RNP complexes from cells." - Cellular and Molecular Biology
- Table of contents (35 chapters)
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Labeling and Purification of RNA Synthesized by In Vitro Transcription
Pages 1-10
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Joining RNA Molecules with T4 DNA Ligase
Pages 11-19
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RNA-Protein Crosslinking with Photoreactive Nucleotide Analogs
Pages 21-33
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The Methylene Blue Mediated Photocrosslinking Method for Dectection of Proteins that Interact with Double-Stranded RNA
Pages 35-47
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Probing RNA-Protein Interactions by Psoralen Photocrosslinking
Pages 49-62
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Table of contents (35 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- RNA'Protein Interaction Protocols
- Editors
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- Susan R. Haynes
- Series Title
- Methods in Molecular Biology
- Series Volume
- 118
- Copyright
- 1999
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59259-676-8
- DOI
- 10.1385/1592596762
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIX, 481
- Topics
