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Gene Therapy Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Paul D. Robbins

    1. University of Pittsburgh, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 7)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Methods for Gene Transfer Using DNA-Adenovirus Conjugates

    • David T. Curiel
    Pages 1-23

  3. Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors

    • Jeffrey S. Bartlett, Richard J. Samulski
    Pages 25-40

  4. Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection

    • Warren S. Pear, Martin L. Scott, Garry P. Nolan
    Pages 41-57

  5. Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers

    • Isabelle Rivière, Michel Sadelain
    Pages 59-78

  6. Development of Replication-Defective Herpes Simplex Virus Vectors

    • David Krisky, Peggy Marconi, William F. Goins, Joseph C. Glorioso
    Pages 79-102

  7. Methods for the Use of Poliovirus Vectors for Gene Delivery

    • Casey D. Morrow, David C. Ansardi, Donna C. Porter
    Pages 103-116

  8. Methods for the Construction of Human Papillomavirus Vectors

    • Saleem A. Khan, Francis M. Sverdrup
    Pages 117-125

  9. Methods for Liposome-Mediated Gene Transfer to the Arterial Wall

    • Elizabeth G. Nabel, Zhi-yong Yang, Hong San, Dianne P. Carr, Gary J. Nabel
    Pages 127-133

  10. Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes

    • Mark A. Findeis, Catherine H. Wu, George Y. Wu
    Pages 135-152

  11. Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium

    • John C. Olsen, Larry G. Johnson, James R. Yankaskas
    Pages 153-168

  12. Methods for Adenovirus-Mediated Gene Transfer to Airway Epithelium

    • John F. Engelhardt
    Pages 169-184

  13. Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung

    • Bruce R. Pitt, Margaret A. Schwarz, Richard D. Bland
    Pages 185-194

  14. In Situ Retrovirus-Mediated Gene Transfer into the Liver

    • Nicolas Ferry, Sophie Branchereau, Jean-Michel Heard, Olivier Danos
    Pages 195-204

  15. Methods for Delivery of Genes to Hepatocytes In Vivo Using Recombinant Adenovirus Vectors

    • Darlene Barr, Mark A. Kay
    Pages 205-212

  16. Methods for Producing High Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells

    • Jane C. Burns, Jiing-Kuan Yee, Alice L. Yu
    Pages 213-221

  17. Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells

    • John W. Belmont, Roland Jurecic
    Pages 223-240

  18. Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes

    • Michel Sadelain
    Pages 241-248

  19. Methods for Retrovirus-Mediated Gene Transfer to CD34+ Enriched Cells

    • Alfred B. Bahnson, Maya Nimgaonkar, Edward D. Ball, John A. Barranger
    Pages 249-263

  20. Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy

    • Sabine A. Eming, Jeffrey R. Morgan
    Pages 265-279
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About this book

In the last few years, significant advances have been made in the area of gene therapy for both genetic and acquired diseases. Improvement in gene transfer methods has allowed for development of gene therapy protocols for the treatment of diverse types of diseases, including metabolic, cardiovas- lar, and autoimmune diseases, as well as cancer. For example, clinical trials for gene therapy of cancer, cystic fibrosis, ADA deficiency, and arthritis, among others, have been initiated in recent years. It is likely that, in the near future, gene therapy will become a common form of treatment for many different types of diseases. Gene therapy takes advantage of recent advances in many areas of molecular and cell biology, including the identification of new the- peutic genes, improvement in both viral and nonviral gene delivery systems, better understanding of gene regulation, and improvement in cell isolation and transplantation. Because of the different and complex techniques involved in achieving successful gene-mediated therapies, it is difficult for scientists to perform all methods required for gene delivery and subsequent in vivo gene expression. This Gene Therapy Protocols volume in the Methods in Molecular Me- cine series provides scientists with detailed methods for working with gene transfer systems, and methods for gene transfer to specific tissue types either in vivo or ex vivo.

Reviews

Such a compilation is of great interest fro researchers who get interested in the protocols utilized in gene transfer and in thier applications. Furthermore, for researchers already involved in gene therapy, this book is a very good reference with a "note section" containing many details and comments, useful to improve current protocols.-ACTA Neurologica Belgica

Editors and Affiliations

  • University of Pittsburgh, USA

    Paul D. Robbins

Bibliographic Information

  • Book Title: Gene Therapy Protocols

  • Editors: Paul D. Robbins

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896034844

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1997

  • eBook ISBN: 978-1-59259-591-4Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 432

  • Number of Illustrations: 44 b/w illustrations

  • Topics: Human Genetics

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