Methods in Molecular Medicine

Gene Therapy Protocols

Editors: Robbins, Paul D. (Ed.)

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About this book

In Gene Therapy Protocols, leading researchers describe in detail all the essential molecular methods for developing gene transfer systems, along with the methods for introducing genes into specific tissue types either in vivo or ex vivo. These easily reproducible methods range from those for specific viral and nonviral delivery systems, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor suppressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.

Gene Therapy Protocols is the first major collection of the methods needed for successful gene delivery and subsequent in vivo gene expression, techniques at the center of the many and significant recent advances in the treatment of both genetic and acquired diseases. It will surely become today's indispensable standard reference source for all scientists working to realize the promises of gene therapy.

Reviews

Such a compilation is of great interest fro researchers who get interested in the protocols utilized in gene transfer and in thier applications. Furthermore, for researchers already involved in gene therapy, this book is a very good reference with a "note section" containing many details and comments, useful to improve current protocols.-ACTA Neurologica Belgica

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Methods for Gene Transfer Using DNA-Adenovirus Conjugates

    Curiel, David T.

    Pages 1-23

  • Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors

    Bartlett, Jeffrey S. (et al.)

    Pages 25-40

  • Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection

    Pear, Warren S. (et al.)

    Pages 41-57

  • Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers

    Rivière, Isabelle (et al.)

    Pages 59-78

  • Development of Replication-Defective Herpes Simplex Virus Vectors

    Krisky, David (et al.)

    Pages 79-102

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-59259-591-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Gene Therapy Protocols
Editors
  • Paul D. Robbins
Series Title
Methods in Molecular Medicine
Series Volume
7
Copyright
1997
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-591-4
DOI
10.1385/0896034844
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XIV, 432
Number of Illustrations
44 b/w illustrations
Topics