Save today: Get 40% off titles in Popular Science!

Methods in Molecular Biology

Protein Sequencing Protocols

Editors: Smith, Bryan John (Ed.)

Free Preview

Buy this book

eBook $79.99
price for USA in USD
  • ISBN 978-1-59259-550-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
About this book

Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples.

Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.

Reviews

"This methodological guide can be recommended to all who are concerned with protein characterization or peptide mapping."-Reviewed by R. Moravec, Biologia Plantarum

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Strategies for Handling Polypeptides on a Microscale

    Pages 1-16

    Smith, Bryan John (et al.)

  • SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing

    Pages 17-24

    Smith, Bryan John

  • Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization

    Pages 25-36

    Dunn, Michael J.

  • Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization

    Pages 37-42

    Dunn, Michael J.

  • Enzymatic Cleavage of Polypeptides

    Pages 43-55

    Smith, Bryan John (et al.)

Buy this book

eBook $79.99
price for USA in USD
  • ISBN 978-1-59259-550-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Protein Sequencing Protocols
Editors
  • Bryan John Smith
Series Title
Methods in Molecular Biology
Series Volume
64
Copyright
1997
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-550-1
DOI
10.1385/0896033538
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 375
Topics