Signal Transduction Protocols

1. Front Matter

   Pages i-xi

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2. The Problems and Pitfalls of Radioligand Binding

   • Mary Keen

   Pages 1-16

3. Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor

   • Jeremy M. Henley, David M. Kirkham

   Pages 17-24

4. Autoradiography of β1-and β2-Adrenoceptors

   • Roger J. Summers, Peter Molenaar

   Pages 25-39

5. In Situ Hybridization

   • Brian J. Morris

   Pages 41-49

6. Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes

   • Marva Sweeney

   Pages 51-61

7. Measurement of Adenylyl Cyclase Activity in Cell Membranes

   • Robert J. Williams, Eamonn Kelly

   Pages 63-77

8. The Measurement of Cyclic AMP Levels in Biological Preparations

   • Stephen P. H. Alexander

   Pages 79-89

9. Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay

   • Jeffrey K. Horton, Peter M. Baxendale

   Pages 91-105

10. Measurement of Cyclic GMP Formation

    • Félix Hernández

    Pages 107-112

11. Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay

    • Kenneth J. Murray

    Pages 113-122

12. Purification and Activation of Cyclic GMP-Dependent Protein Kinase

    • Takayoshi Kuno, Hideyuki Mukai

    Pages 123-127

13. The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity

    • Mohammed Shahid, C. David Nicholson

    Pages 129-150

14. Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC)

    • Stephen Jenkinson

    Pages 151-165

15. Mass Measurement of Key Phosphoinositide Cycle Intermediates

    • R. A John Challiss

    Pages 167-176

16. Measurement of Phospholipase C Activity in Brain Membranes

    • Enrique Claro, Elisabet Sarri, Fernando Picatoste

    Pages 177-188

17. Measurement of Diacylglycerol

    • Michael R. Boarder, John R Purkiss

    Pages 189-202

18. Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2

    • Philip A Iredale, John M. Dickenson

    Pages 203-213

19. Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3

    • Robert A. Wilcox, James Strupish, Stefan R. Nahorski

    Pages 215-227

20. Single-Cell Calcium Imaging

    • Katrina A. Marsh

    Pages 229-238

As our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches.

...this well-organized volume is clearly among the most viable protocol books published in this field and will turn out to be a helpful tool for many laboratories.-FEBS Letters
...well organzied...up to date...should be in each laboratory or at least in your department library for easy reference. -Biopharm

• Queen’s Medical Centre, University of Nottingham, UK

  David A. Kendall, Stephen J. Hill

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