Methods in Molecular Biology

PCR Detection of Microbial Pathogens

Editors: Sachse, Konrad, Frey, Joachim (Eds.)

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About this book

Because the number of published PCR procedures is already large and rising steadily, there is a need to critically evaluate and eventually standardize its methodology so that laboratory diagnosticians can select optimal techniques for their own work. In PCR Detection of Microbial Pathogens, hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. Described in step-by-step detail to ensure successful experimental results, these proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis. Each article details a validated technique that explains the principles involved, provides stepwise instructions and a list of materials, and includes notes describing problems that may arise, as well as possible alternative procedures.
Authoritative and easy-to-use, PCR Detection of Microbial Pathogens offers microbiologists, veterinary diagnosticians, and food analysts powerful state-of-the-art tools designed not only to expand their knowledge of the potential of DNA-based diagnostic methods, but also to help select those techniques best suited to their specific practical needs.

Reviews

"...PCR Detection of Microbial Pathogens is useful, comprehensive and practical guide for everyone who wants to use PCR for detection of the above mentioned pathogens and, at the same time, an inspiration for development of new PCR detection systems." -Folia Microbiologica

Table of contents (22 chapters)

Table of contents (22 chapters)

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-1-59259-344-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
PCR Detection of Microbial Pathogens
Editors
  • Konrad Sachse
  • Joachim Frey
Series Title
Methods in Molecular Biology
Series Volume
216
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-344-6
DOI
10.1385/1592593445
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 336
Topics