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Methods in Molecular Biology

RT-PCR Protocols

Editors: King, Nicola, O’Connell, Joe (Eds.)

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About this book

RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances.
Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies.

Reviews

"...RT-PCR succeeds in its goal to provide the reader with useful applications relevant to the "wider research community." -Clinical Chemistry

"It is clearly written and can be easily understood by the readers. It is recommended for students, geneticists, immunologists and other specialists in biomedicine." -Folia Microbiologica

Table of contents (27 chapters)

Table of contents (27 chapters)

Buy this book

eBook $74.99
price for USA in USD
  • ISBN 978-1-59259-283-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
RT-PCR Protocols
Editors
  • Nicola King
  • Joe O’Connell
Series Title
Methods in Molecular Biology
Series Volume
193
Copyright
2002
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-283-8
DOI
10.1385/159259283X
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 382
Topics

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