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Methods in Molecular Biology

Adhesion Protein Protocols

Editors: Dejana, Elisabetta, Corada, Monica (Eds.)

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About this book

In Adhesion Protein Protocols, an international corps of expert investigators describe their optimized techniques for both the identification of new cell adhesion proteins and the characterization of novel adhesive structures. Their cutting edge methods will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. The protocols described here constitute a guide to some of the most important assays in cell biology, promising significant results not only to those interested in adhesion processes, but also to scientists from many different disciplines.

Adhesion Protein Protocols is the first collection of methodologies devoted entirely to the study of adhesion molecules. By illuminating these molecules and the possibilities for manipulating them, the new experimental strategies collected here offer powerful approaches to the regulation of immunity, inflammation, tissue remodeling, and embryonic development. The book will become the standard source for those wishing to discover new adhesion molecules and to characterize their biological activity.



Reviews

"I think this book presents an excellent manual not only for beginning graduate students, who can find a variety of basic techniques, but also for experienced researchers who are new in the field of endothelial biology. . .a very useful manual, containing almost every technique dealing with the cell adhesion properties of the endothelium."-Doody's Health Sciences Book Review Journal

"Dejana and Corada have assembled a wide range of chapters covering methods used for the study of adhesion proteins. The individual chapters are generally well written, and most of the methods that would be expected in such a volume are covered."-Cell Biology International

Table of contents (24 chapters)

  • Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules

    CabaƱas, Carlos (et al.)

    Pages 1-9

  • Epitope Mapping

    DeLisser, Horace M.

    Pages 11-20

  • Sequencing of Antibodies

    Jarrin, Annie (et al.)

    Pages 21-28

  • Purification Through Affinity Chromatography and Microsequencing

    Garlanda, Cecilia

    Pages 29-37

  • Functional Analysis of Cell Adhesion Molecules

    Simmons, David L.

    Pages 39-63

Buy this book

eBook $79.99
price for USA in USD (gross)
  • ISBN 978-1-59259-258-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Adhesion Protein Protocols
Editors
  • Elisabetta Dejana
  • Monica Corada
Series Title
Methods in Molecular Biology
Series Volume
96
Copyright
1999
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-258-6
DOI
10.1385/1592592589
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 229
Number of Illustrations and Tables
4 b/w illustrations
Topics