Overview
- Editors:
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Elisabetta Dejana
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Vascular Biology Laboratory, Istituto di Richerche Farmacologie “Mario Negri”, Milano, Italy
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Monica Corada
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Vascular Biology Laboratory, Istituto di Richerche Farmacologie “Mario Negri”, Milano, Italy
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Table of contents (24 protocols)
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- Carlos Cabañas, Francisco Sánchez-Madrid
Pages 1-9
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- Annie Jarrin, Annie Andrieux
Pages 21-28
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- Amy S. Woodard, Lucia R. Languino
Pages 65-76
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- Thomas Moll, Dietmar Vestweber
Pages 77-84
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- Pier Carlo Marchisio, Livio Trusolino
Pages 85-92
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- Antonella Stoppacciaro, Luigi P. Ruco
Pages 93-106
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- Saverio Francesco Retta, Piercarlo Ferraris, Guido Tarone
Pages 119-124
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- Saverio Francesco Retta, Massimo Ternullo, Guido Tarone
Pages 125-130
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- Jeanne-Marie Kiely, Francis W. Luscinskas, Michael A. Gimbrone Jr.
Pages 131-136
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- Douglas J. Goetz, Daniel M. Greif, Jian Shen, Francis W. Luscinskas
Pages 137-145
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- Renato G. S. Chiarivi, Raffaella Giavazzi
Pages 147-151
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- Andrea Remuzzi, Raffaella Giavazzi
Pages 153-157
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- Philip G. de Groot, Martin J. W. IJsseldijk, Jan J. Sixma
Pages 159-170
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- Paola Allavena, Aldo Del Maschio
Pages 171-176
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About this book
Adhesion molecules are of fundamental importance in the regulation of immunity, inflammation, tissue remodeling, and embryonic development. They comprise different families of homologous proteins, such as selectins, integrins, cadherins, and immunoglobins. In addition, beyond these groups, other str- tures with adhesive properties, such as proteoglycans, occludin, and CD44, have been characterized recently. An understanding of the type and characteristics of adhesive molecules expressed by the different cell types and the possibility of manipulating their activity promises considerable clinical potential. Antibodies, small peptidic and nonpeptidic molecules, have recently been used to inhibit thrombosis by blocking platelet aggregation or inflammation through inhibition of leukocyte infiltration and adhesion. Inhibitors of adhesive molecules are used in expe- mental systems for the study of tumor growth and dissemination. Among major goals in the field are the identification of new members of the known adhesive protein families and of independent new adhesive structures. After structural characterization, even more demanding is the study of the biological activity of the new proteins, and the development of simple, rapid tests for the screening of possible inhibitors. In this regard, the production of such reagents as fragments and antibodies would help define the structure–function relati- ship of individual proteins. Data available in the literature show the complexity of the adhesive process and how different molecular epitopes might contribute to the adhesive properties of a single structure. Finally, a new area of investi- tion is the characterization of the intracellular signaling cascade triggered by the engagement of transmembrane adhesive proteins.
Reviews
"I think this book presents an excellent manual not only for beginning graduate students, who can find a variety of basic techniques, but also for experienced researchers who are new in the field of endothelial biology. . .a very useful manual, containing almost every technique dealing with the cell adhesion properties of the endothelium."-Doody's Health Sciences Book Review Journal
"Dejana and Corada have assembled a wide range of chapters covering methods used for the study of adhesion proteins. The individual chapters are generally well written, and most of the methods that would be expected in such a volume are covered."-Cell Biology International
