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Methods in Molecular Biology

Immunochemical Protocols

Editors: Pound, John (Ed.)

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  • ISBN 978-1-59259-257-9
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About this book

This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates.

The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.

Reviews

"...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques."
-Microbiology Today

Table of contents (47 chapters)

Table of contents (47 chapters)
  • Production of Polyclonal Antisera

    Pages 1-4

    Green, Jonathan A. (et al.)

  • Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen

    Pages 5-13

    Diano, Monique (et al.)

  • Production and Characterization of Antibodies Against Synthetic Peptides

    Pages 15-22

    Hancock, David C. (et al.)

  • Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins

    Pages 23-37

    Dean, Christopher J.

  • A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies

    Pages 39-56

    Goodall, Margaret

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-1-59259-257-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Immunochemical Protocols
Editors
  • John Pound
Series Title
Methods in Molecular Biology
Series Volume
80
Copyright
1998
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-257-9
DOI
10.1007/978-1-59259-257-9
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 508
Topics

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