Basic DNA and RNA Protocols

Hybridization and Competition Hybridization of Southern Blots

Pages 31-39

Kelsell, Rosemary E.

Utilization of DNA Probes with Digoxigenin-Modified Nucleotides in Southern Hybridizations

Pages 41-51

Helentjaris, Tim (et al.)

The Preparation of Fluorescein-Labeled Nucleic Acid Probes and Their Detection Using Alkaline Phosphatase-Catalyzed Dioxetane Chemiluminescence

Pages 53-63

Cunningham, Martin (et al.)

Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay

Pages 65-66

Cunningham, Martin

The Preparation of Horseradish Peroxidase Labeled Nucleic Acid Probes and Their Detection Using Enhanced Chemiluminescence

Pages 67-75

Harvey, Bronwen (et al.)

3′-End Labeling of Oligonucleotides with Fluorescein-11-dUTP and Enhanced Chemiluminescent Detection

Pages 77-82

Cunningham, Martin (et al.)

The Preparation of Riboprobes

Pages 83-91

Belin, Dominique

Native Polyacrylamide Gel Electrophoresis

Pages 93-96

Harwood, Adrian J.

Use of Silver Staining to Detect Nucleic Acids

Pages 97-103

Mitchell, Lloyd G. (et al.)

End-Labeling of DNA Fragments

Pages 105-110

Harwood, Adrian J.

Northern Blot Analysis

Pages 113-128

Krumlauf, Robb

RNA Slot Blotting

Pages 129-130

Murphy, David

The RNase Protection Assay

Pages 131-136

Belin, Dominique

Primer Extension Analysis of mRNA

Pages 137-145

Leonard, Mark W. (et al.)

S1 Mapping Using Single-Stranded DNA Probes

Pages 147-153

Viville, Stéphane (et al.)

Nonradioactive In Situ Hybridization for Cells and Tissues

Pages 155-167

Durrant, Ian

In Vitro Packaging of DNA

Pages 171-175

Dale, Jeremy W. (et al.)

Construction of Mammalian Genomic Libraries Using λ Replacement Vectors

Pages 177-190

Bateson, Alan N. (et al.)

The Production of Double-Stranded Complementary DNA for Use in Making Libraries

Pages 191-198

Mayall, Steve (et al.)

Construction of cDNA Libraries

Pages 199-209

Burrell, Michael M.

Screening λ Libraries

Pages 211-218

Harwood, Janet C. (et al.)

Subcloning Strategies and Protocols

Pages 221-235

Taghian, Danielle Gioioso (et al.)

Purification of DNA Fragments from Agarose Gels Using Glass Beads

Pages 237-240

Joly, Etienne

Transformation of E. coli

Pages 241-247

Tomley, Fiona M.

Transformation of Bacteria by Electroporation

Pages 249-256

Drury, Lucy

Preparation of Plasmid DNA by Alkaline Lysis

Pages 257-263

Joly, Etienne

The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA

Pages 265-267

Harwood, Adrian J.

Plasmid Preparations with Diatomaceous Earth

Pages 269-272

Machesky, Laura

Polymerase Chain Reaction

Pages 275-292

Delidow, Beverly C. (et al.)

Inverse Polymerase Chain Reaction

Pages 293-301

Hartl, Daniel L. (et al.)

Polymerase Chain Reaction with Degenerate Oligonucleotide Primers to Clone Gene Family Members

Pages 303-312

Preston, Gregory M.

Cloning PCR Products Using T-Vectors

Pages 313-324

Trower, Michael K. (et al.)

Direct Radioactive Labeling of Polymerase Chain Reaction Products

Pages 325-327

McDaniel, Tim (et al.)

A Rapid PCR-Based Colony Screening Protocol for Cloned Inserts

Pages 329-333

Trower, Michael K.

Use of Polymerase Chain Reaction to Screen Phage Libraries

Pages 335-339

Yu, Lei (et al.)

Cloning into M13 Bacteriophage Vectors

Pages 343-348

Yu, Qingzhong

Ordered Deletions Using Exonuclease III

Pages 349-357

Clark, Denise (et al.)

M13 Phage Growth and Single-Stranded DNA Preparation

Pages 359-362

Tomley, Fiona M.

Preparation of ssDNA from Phagemid Vectors

Pages 363-366

Trower, Michael K.

A Rapid Plasmid Purification Method for Dideoxy Sequencing

Pages 367-371

Grifln, Annette M. (et al.)

DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase

Pages 373-387

Fuller, Carl W. (et al.)

Pouring Linear and Buffer-Gradient Sequencing Gels

Pages 389-392

Littlebury, Paul

Electrophoresis of Sequence Reaction Samples

Pages 393-401

Bankier, Alan T.

Direct Sequencing of PCR Products

Pages 403-412

Harwood, Janet C. (et al.)

Thermal Cycle Dideoxy DNA Sequencing

Pages 413-423

Slatko, Barton E.

Using the Automated DNA Sequencer

Pages 425-439

Du, Zijin (et al.)

Terminal Labeling of DNA for Maxam and Gilbert Sequencing

Pages 441-446

Pichersky, Eran

DNA Sequencing by the Chemical Method

Pages 447-451

Pichersky, Eran

Site-Directed Mutagenesis of Double-Stranded Plasmids, Domain Substitution, and Marker Rescue by Comutagenesis of Restriction Enzyme Sites

Pages 455-468

Nickoloff, Jac A. (et al.)

A Protocol for Site-Directed Mutagenesis Employing a Uracil-Containing Phagemid Template

Pages 469-476

Trower, Michael K.

In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System

Pages 477-484

Olliver, Louise (et al.)

In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System

Pages 485-490

Olliver, Louise (et al.)

One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin

Pages 491-510

Crowe, Joanne (et al.)