Methods in Molecular Biology

Embryonic Stem Cells

Methods and Protocols

Authors: Turksen, Kursad

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About this book

Embryonic stem (ES) cells have significant potential in basic studies designed to better understand how different cells and tissues in the body are formed, as well as for generating unlimited numbers of cells for transplantation, drug delivery, and drug testing. In Embryonic Stem Cells: Methods and Protocols, Kursad Turksen and a panel of international experts describe their most productive methods for using ES cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail by the investigators who developed them, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries. There are also advanced techniques for the generation of antibodies against very rare antigens and for the identification and characterization of proteins and protein interactions. Additional studies of the ES cell cycle and apoptosis, as well as protocols for the use of ES cells to generate diverse cell and tissue types, complete this collection of readily reproducible methods. Many of the techniques have already been shown to have tremendous utility with ES cells and their differentiated progeny.
Authoritative and state-of-the-art, this unique first collection of protocols for the study of ES cells, Embryonic Stem Cells: Methods and Protocols, will prove an invaluable resource not only for those generally interested in cell and developmental biology, but also for those actively using, or planning to use, ES cells to study fate choices and specific lineages.

Reviews

"The book could be used effectively by experienced or novice researchers in the field since the protocols are written clearly and concisely. There are 35 brief chapters in this book by over 80 authors who are experienced and knowledgeable researchers in the field...Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing ES cells from a variety of experimental animals. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to culture ES cells and/or apply the specialized techniques for studying them. There is an adequate index. This book is intended and written as a laboratory guide manual. It would be quite useful for anyone doing bench research on ES cells." -Doody's Health Sciences Book Review Journal

"...this book provides a good overview of work being done in the development of protocols for culture, controlled differentiation and manipulation of ES cells" - Newsletter of the British Society of Developmental Biology

"Embryonic Stem Cells Methods and Protocols is one of the most useful books I have ever read in this field, and the awesome note at he end of each chapter makes it a great work. It represents good value for money and should be useful as a reference book." - Cell Biology International

"...a valuable collection of up-to-date protocols written by leading experts in the field. It is well written and clearly structured. The volume appears highly valuable for any scientist who wants to make a start in this exciting field but also for experienced ES cell researchers who want to widen their repertoire. In addition, the protocols involving hematopoietic, adipocyte and vascular differentiation could easily be adopted by diabetologists to promote our understanding of cell systems which are of special interest in diabetes research." - Diabetologia

"This book provides a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research." -Molecular Biotechnology

Buy this book

eBook $99.00
price for USA in USD (gross)
  • ISBN 978-1-59259-241-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Embryonic Stem Cells
Book Subtitle
Methods and Protocols
Authors
Series Title
Methods in Molecular Biology
Series Volume
185
Copyright
2002
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-241-8
DOI
10.1385/1592592414
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 499
Number of Illustrations and Tables
187 b/w illustrations, 54 illustrations in colour
Topics