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Methods in Molecular Biology

Genomics Protocols

Editors: Starkey, Michael P., Elaswarapu, Ramnath (Eds.)

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About this book

Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome.
Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.

Reviews

"The two editors of this volume have invited contributors from a variety of authors to write a laboratory manual dealing exclusively with the application of genomic methods in eukaryotes....Each chapter is presented in a standardized fashion, beginning with an introduction followed by a list of all the materials needed (including the machines and software) a detailed experimental protocol, notes (troubleshooting), and a list of references....it will be of value for those requiring step-by-step "cookbook" procedures for some of the methodologies described, and this definitive and authorative volume belongs in the library of every quality research institution where genomics is performed." - CHEMBIOCHEM

"...a solid introduction to the basic techniques involved in the process of disease gene identification...should prove to be useful to those scientists who wish to expand their skills in the field." -Biotech Software & Internet Report

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse

    Pages 1-9

    Lyons, Paul A.

  • Genetic Analysis of Complex Traits

    Pages 11-27

    Bryant, Stephen P. (et al.)

  • Sequence-Based Detection of Single Nucleotide Polymorphisms

    Pages 29-35

    Nickerson, Deborah A. (et al.)

  • Genomic Mismatch Scanning for the Mapping of Genetic Traits

    Pages 37-46

    Mirzayans, Farideh (et al.)

  • Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization

    Pages 47-55

    Hermsen, Mario A. J. A. (et al.)

Buy this book

eBook $99.00
price for USA in USD (gross)
  • ISBN 978-1-59259-235-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Genomics Protocols
Editors
  • Michael P. Starkey
  • Ramnath Elaswarapu
Series Title
Methods in Molecular Biology
Series Volume
175
Copyright
2001
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-235-7
DOI
10.1385/159259235X
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 538
Number of Illustrations
113 b/w illustrations, 5 illustrations in colour
Topics

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