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Adipose Tissue Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Gérard Ailhaud

    1. Université de Nice-Sophia Antipolis, Nice, France

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Part of the book series: Methods in Molecular Biology (MIMB, volume 155)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Choosing an Adipose Tissue Depot for Sampling

    • Louis Casteilla, Luc Pénicaud, Béatrice Cousin, Denis Calise
    Pages 1-19

  3. Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue

    • Saverio Cinti, M. Cristina Zingaretti, Raffaella Cancello, Enzo Ceresi, Plinio Ferrara
    Pages 21-51

  4. Confocal Microscopy of Adipocytes

    • Daniela Malide
    Pages 53-64

  5. Cellularity Measurements

    • Mario DiGirolamo, Jacqueline B. Fine
    Pages 65-75

  6. Subcellular Fractionation of Adipocytes and 3T3-L1 Cells

    • Hans-Georg Joost, Annette Schürmann
    Pages 77-82

  7. Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies

    • Hubert Vidal
    Pages 83-88

  8. Glycogen Synthase Activity in Adipose Tissue

    • Heidi K. Ortmeyer
    Pages 89-96

  9. Assays of Lipolytic Enzymes

    • Cecilia Holm, Gunilla Olivecrona, Malin Ottosson
    Pages 97-119

  10. Assays of Lipogenic Enzymes

    • Raymond Bazin, Pascal Ferré
    Pages 121-127

  11. Assays of Adrenergic Receptors

    • Christian Carpéné
    Pages 129-140

  12. Transfection of Adipocytes and Preparation of Nuclear Extracts

    • Isabelle Dugail
    Pages 141-146

  13. Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes

    • Nada A. Abumrad
    Pages 147-155

  14. Assays of Glucose Entry, Glucose Transporter Amount, and Translocation

    • Jean-François Tanti, Mireille Cormont, Thierry Grémeaux, Yannick Le Marchand-Brustel
    Pages 157-165

  15. Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes

    • Eva Degerman, Svante Resjö, Tova Rahn Landström, Vincent Manganiello
    Pages 167-180

  16. Measurements of Glucose Conversion to its Metabolites

    • Mario DiGirolamo
    Pages 181-192

  17. Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes

    • Jan Nedergaard
    Pages 193-196

  18. Culture of Adipose Tissue and Isolated Adipocytes

    • Susan K. Fried, Naima Moustaid-Moussa
    Pages 197-212

  19. Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines

    • Barbara Cannon, Jan Nedergaard
    Pages 213-224

  20. Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue

    • Raymond Négrel, Christian Dani
    Pages 225-237
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About this book

Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.

Editors and Affiliations

  • Université de Nice-Sophia Antipolis, Nice, France

    Gérard Ailhaud

Bibliographic Information

  • Book Title: Adipose Tissue Protocols

  • Editors: Gérard Ailhaud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592317

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • eBook ISBN: 978-1-59259-231-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 334

  • Topics: Cell Biology

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