Methods in Molecular Biology

DNA'Protein Interactions

Principles and Protocols

Editors: Moss, Tom (Ed.)

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About this book

DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination.
Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.

Reviews

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology

"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science

Table of contents (43 chapters)

Table of contents (43 chapters)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-208-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
DNA'Protein Interactions
Book Subtitle
Principles and Protocols
Editors
  • Tom Moss
Series Title
Methods in Molecular Biology
Series Volume
148
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-208-1
DOI
10.1385/1592592082
Series ISSN
1064-3745
Edition Number
2
Number of Pages
656
Number of Illustrations
146 b/w illustrations
Topics