Springer Protocols Handbooks

Protocols for Neural Cell Culture

Editors: Fedoroff, Sergey, Richardson, Arleen (Eds.)

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About this book

In Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures.
Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.

Reviews

"In addition to covering standard protocols in well-established culture systems, this is one of the first manuals to include a chapter on protocols to culture neural stem cells. Another unique feature is the inclusion of pictures to facilitate the dissection of particular brain areas, which will be of great help for workers not completely familiar with different aspects of developmental neuroanatomy. . .This is a valuable tool for "culturists" of neural cells. This edition updates protocols for the culturing of neural cell types traditionally used in laboratories studying different aspects of neurobiology and it provides easy-to follow instructions for setting up and maintaining cultures of progenitor cells that will be used in many laboratories in years to come."-Doody's Health Science Book review Journal

"...presents a series of excellent neural tissue culture protocols designed for bench use."
-Trends in Cell Biology

"...a valuable resource that will find its place on many laboratory shelves..."
-Trends in Biotechnology

"...the recipes are comprehensive and easy to follow."
-Trends in Neurosciences

"...indispensable for every project in neuroscience."
-Doody's Health Sciences Book Review Journal

Table of contents (27 chapters)

Table of contents (27 chapters)

Buy this book

eBook $74.99
price for USA in USD (gross)
  • ISBN 978-1-59259-207-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Protocols for Neural Cell Culture
Editors
  • Sergey Fedoroff
  • Arleen Richardson
Series Title
Springer Protocols Handbooks
Copyright
2001
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-59259-207-4
DOI
10.1385/1592592074
Series ISSN
1949-2448
Edition Number
3
Number of Pages
XXII, 262
Topics