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Methods in Molecular Biology

Transgenesis Techniques

Principles and Protocols

Editors: Clarke, Alan R. (Ed.)

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About this book

The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.
Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.

Reviews

From Reviews of the First Edition
"...a must in any laboratory..." -Cellular and Molecular Biology

"...should be useful to anyone currently involved in using transgenesis as a technique..."
-Journal of Heredity
Second Edition:

"...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates." - Human Genetics

"It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this." -Cloning and Stem Cells

Table of contents (15 chapters)

Table of contents (15 chapters)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-59259-178-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Transgenesis Techniques
Book Subtitle
Principles and Protocols
Editors
  • Alan R. Clarke
Series Title
Methods in Molecular Biology
Series Volume
180
Copyright
2002
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-178-7
DOI
10.1385/1592591787
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 351
Topics